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Ion transport mechanism of marginal cells and basal cells dissociated form the stria vascularis in the inner ear

Research Project

Project/Area Number 07671864
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKochi Medical School

Principal Investigator

TAKEUCHI Shunji  Kochi Medical School, Physiology, Research Associate, 医学部, 助手 (50188158)

Co-Investigator(Kenkyū-buntansha) IRIMAJIRI Akihiko  Kochi Medical School, Physiology, Professor, 医学部, 教授 (30025605)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsInner ear / Stria vascularis / Marginal cell / Basal cell / Ion transport
Research Abstract

(1) The basolateral membrane of isolated strial marginal cells was probed for conductive pathways by patch-clamp technique. Two types of channels, a nonselective cation channel (25 pS) and a Cl channel, (80 pS) were identified.
(2) Using the whole-cell patch-clamp technique, Cl-selective currents manifested by strial marginal cells were investigated. A large Cl conductance of 100 pS/cell was found from nonswollen cells in an isotonic solution.
(3) The whole-cell Cl conductance of the marginal cell varied in parallel with cell volume. The permeability sequence of this current was SCN>Br=Cl>F>NO_3>I>gluconate.
(4) Maxi-K channels were identified in the plasma membrane of basal cells. The physiological significance of these channels have not been determined.
(5) Effects of K channel blockers on the positive endocochlear potential (EP) of guinea pigs maintained by vascular perfusion were studied. The EP levels was reversibly blocked by Ba^<2+>, quinine and verapamil in the perfusate, but not by tetraethylammonium or 4-aminopyridine. These findings indicate an important role for a K conductance in the generation of EP.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Shunji Takeuchi et al.: "Ion channels in baselateral membrane of marginal cells dissociated from gerbil stria vascularis" Hearing Research. 83. 89-100 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunji Takeuchi,A.Irimajiri: "A novel,volume-correlated Cl^- conductance in marginal cells dissociated from the stria vascularis of gerbils" Journal of Membrane Biology. 150. 47-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunji Takeuchi,A.Irimajiri: "Maxi-K^+ channel in plasma membrane of basal cells dissociated from the stria vascularis of gerbils" Hearing Research. 95. 18-25 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunji Takeuchi et al.: "Intravascularly applied K^+ channel blockers suppress differently the positive endocochlear potential main tained by vascular perfusion" Hearing Research. 101. 181-185 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi, S., Kakigi, A., Takeda, T., Saito, H., Irimajiri, A: "Intravascularly applied K^+-channel blockers suppress differently the positive endocochlear potential maintained by vascular perfusion." Hearing Research. 101. 181-185 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi, S., Irimajiri, A: "Maxi-K^+ channel in plasma membrane of basal cells dissociated from the stria vascularis of gerbils." Hearing Research. 95. 18-25 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi, S., Irimajiri, A: "A novel, volume-correlated Cl^- conductance in marginal cells dissociated from the stria vascularis of gerbils" Journal of Membrane Biology. 150. 47-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi, S., Ando, M., Kozakura, K., Saito, H., Irimajiri, A: "Ion channels in basolateral membrane of marginal cells dissociated from gerbil stria vascularis." Hearing Research. 83. 89-100 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunji Takeuchi et al.: "Ion channels in basolateral membrane of marginal cells dissociated from gerbil stria vascularis" Hearing Research. 83. 89-100 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shunji Takeuchi,A.Irimajiri: "A novel,volume-correlated Cl^- conductance in marginal cells dissociated from the stria vascularis of gerbils" Journal of Membrane Biology. 150. 47-62 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shunji Takeuchi,A.Irimajiri: "Maxi-K^+ channel in plasma membrane of basal cells dissociated from the stria vascularis of gerbils" Hearing Research. 95. 18-25 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shunji Takeuchi et al.: "Intravascularly applied K^+-channel blockers suppress differently the positive endocochlear potential mantained by vascular perfusion" Hearing Research. 101. 181-185 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shunji Takeuchi: "A novel,volume-correlated Cl^-conductance in marginal cells dissociated from the stria vascularis of gerbils" Journal of Membrane Biology. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] Shunji Takeuchi: "Maxi・K^+ channel in plasma membrane of basal cells dissociated from the stria vascularis of gerbils" Hearing Research. (印刷中).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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