• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The Etiology of Endolymphatic Hydrops-Role of Spiral Ligament and Endolymphatic Sac-

Research Project

Project/Area Number 07671868
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionOita Medical University

Principal Investigator

ICHIMIYA Issei  Dept.of Otolaryngology, Oita Medical University, Assistant Professor, 医学部, 講師 (70223112)

Co-Investigator(Kenkyū-buntansha) SUZUKI Masashi  Dept.of Otolaryngology, Oita Medical University, Associate Professor, 医学部, 助教授 (60211314)
MOGI Goro  Dept.of Otolaryngology, Oita Medical University, Professor, 医学部, 教授 (20035190)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsendolymphatic hydrops / spiral ligament / endolymphatic sac / keyhole limpet hemocyanin / immunohistochemistry / connexin 26 / fibrocytes / extracellular matrix / vimentin / S-100 / connexin26 / proteoglycan / collagen / ラセン靱帯 / Na,K-ATPase / gap junction / fibrinogen / keyhole lympet hemocyanin / 肺炎球菌 / 免疫傷害 / 血液-内耳関門 / 血管条 / Na^+,K^+-ATPase / ギャップ・ジャンクション / エンドトキシン / ABR
Research Abstract

1)Labyrinthitis was induced by inoculation of keyhole limpet hemocyanin into the scala tympani of systemically sensitized guinea pigs, and inner ears were immunostained. Spiral ligament, spiral limbus, and blood vessels including the spiral modiolar vein were diffusely positive for IgG and albumin. Immunoreactivity for connexin 26 and Na-K-ATPase was decreased in the fibrocytes of the spiral ligament, however, endolymphatic hydrops was not induced.
2)Changes in cochleas with experimental otitis media were investigated in mice. Otitis media was induced in all of the animals after the inoculation of Streptococcus pneumoniae into the middle ear cavity, and some of them showed inflammatory cells in the cochlea. Although other changes, including endolymphatic hydrops, were not obvious, cochleas showed positive immunostaining for fibrinogen. Immunostaining for connexin 26 was slightly decreased in the spiral ligament, accompanied by fibrinogen staining.
3)Endolymphatic sacs of the guinea pigs … More were ablated to induce endolymphatic hydrops and processed for immunohistochemistry. Staining of type I fibrocytes in the spiral ligament, which are positive for vimentin and connexin 26, was decreased. However, immunostaining for extracellular matrix in the spiral ligament, which are positive for chondroitin 4-sulfate proteogly can and type II collagen, showed no changes.
Because endolymphatic hydrops is not always obseved with the changes in the fibrocytes of the spiral ligament, it is suggested that the spiral ligament is not responsible for the production of endolymphatic hydrops. However, spiral ligament appears to be responsible for the cochlear malfunction, and immunostaining for fibrinogen or other blood constituents suggests that the blood-labyrinth barrier could disrupt in the spiral ligament by labyrinthitis or otitis media. The constant staining for the extracellular matrix with the experimental endolymphatic hydrops raises the possibility that changes in the spiral ligament has been underestimated by routine otopathologic observations. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 一宮 一成 他: "内耳免疫傷害動物の病理組織" Otol Jpn. 6. 170-176 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 一宮 一成 他: "鼓膜における免疫担当細胞の分布-マウスによる免疫組織学的検討-" Otol Jpn. 6. 446 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I, et al.: "Immunological potential of the tympanic membrane.Observation under normal and inflammatory conditions" Am J Otolaryngol. 18. 165-172 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 一宮 一成 他: "実験的中耳炎の蝸牛に及ぼす影響" Otol Jpn. 7. 554 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I, et al.: "Changes in the lateral wall of the cochlea after experimental otitis media" Proc Sendai Symposium. 7. 43 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I, et al.: "Changes in Immunostaining of Inner Ears after Antigen Challenge into the Scala Tympani" Laryngoscope. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I,et al.: "Immunology of the tympanic membrane." Acta Oto-rhino-laryngologica Belg. 49. 121-125 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I,et al.: "Immunohistochemical observation of the inner ear with experimental immune injury." Otol Jpn.6. 170-176 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I,et al.: "Immunological potential of the tympanic membrane. Observation under normal and inflammatory conditions." Am J Otolaryngol. 18. 165-172 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I,et al.: "Changes in immunostaining of inner ears after antigen challenge into the scala" tympani. Laryngoscope. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I,et al.: "Changes in the lateral wall of the cochlea after experimental otitis media." Proceedings of Sendai Symposium '97 on Ear Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ichimiya I,et al.: "Immunological potential of the tympanic membrane.Observation under normal and inflammatory conditions" Am J Otolaryngol. 18. 165-172 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ichimiya I,et al.: "Changes in Immunostaining of Inner Ears after Antigen Challenge into the Scala Tympani" Laryngoscope. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Ichimiya I,et al.: "Changes in the lateral wall of the cochlea after experimental otitis media" Proc Sendai Symposium. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 一宮一成 他: "内耳免疫傷害動物の病理組織" Otology Japan. 6(3). 170-176 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 一宮一成 他: "鼓膜における免疫担当細胞の分布-マウスによる免疫組織学的検討-" Otology Japan. 6(4). 446 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichimiya I, et al: "Immunological potential of the tympanic membrance. immunohistochemical observation of mice under normal and inflammatory conditions" American Journal of Otolaryngology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木正志 他: "Expression of intercellular adhesion molecule-1 during inner ear inflammation" Annals of Otology,Rhinology & Laryngology. 104. 69-75 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 一宮一成 他: "Immunology of the tympanic membrane" Acta Oto-Rhino-Laryngologica Belgica. 49. 121-125 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 一宮一成: "内耳の免疫現象-内耳免疫傷害動物の病理組織-" Otology Japan. 5. 503-503 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi