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Studies of the chemotherapy for the hed and neck carcinoma.-Development of the second line chemotherapy for CDDP resistant multicellular tumor spheroids-.

Research Project

Project/Area Number 07671884
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionJUNTENDO UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

KOHNO Naoyuki  Juntendo univ.school of med.oto-rhino-laryngology.Associate Professor, 医学部, 助教授 (20118946)

Co-Investigator(Kenkyū-buntansha) NAKAZAWA Eiko  Juntendo univ.school of med.oto-rhino-laryngology.Research Associate, 医学部, 助手 (60245751)
NAKAMURA Shoutaro  Juntendo univ.school of med.oto-rhino-laryngology.Research Associate, 医学部, 助手 (70245752)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsHead and Neck cancer / Drug response assay / Multicellular tumor spheroids(MTS) / CDDP refractory MTS / Paclitaxl(Taxol) / MTS from biopsy material / Solid tumor model / Chemotherapy / 頭頚部癌 / 薬剤感受性試験 / コロニー形成率 / 願化学療法 / 併用療法 / 扁平上皮癌
Research Abstract

Multicellular tumor spheroids (MTS) have certain characteristics similar de novo solid tumors. They contain such extracellular matrix as fibronectin, laminin, collagen and hyaluronic acid, comprise a chronically hypoxic cell population in the core and show heterogeneous cell cycle times. Comparison of the three-dimensional culture methods (MTS) with two-dimensional culture methods (monolayr) has shown differences in the behavior of chemotherapeutic drug responses. Practical applications of MTS is significant for drug sensitivity test.
We studied the cell kill effects of paclitaxl for CDDP resistant HEp-2 and KB MTS.The cell lethality were dose and exposure time related. CDDP resistant MTS are sensitive to paclitaxl. Time schedule for the combination of CDDP and paclitaxdl were also studied. Paclitaxl exposed first followed by CDDP were the most efficaciaous combinaton. Clinical application as the second line chemotherapy, paclitaxl exposed first followed by CDDP were warranted.
Biopsy material from human head and neck squamous cell carcinoma was cut with scalpels into pieces less than 0.5 mm in diameter and maintained in agar plate by liquid overlay culture technique.Using this method, we established the new technique of MTS formation from biopsy material. Untill now, 10 MTS were made from sequential biopsy material. A head and neck tumor is composed not only of tumor cells, but also of normal mesenchymal, endothelial, lymphocyte, macrophages and fibroblast. Therefore heterogeneous cultures of tumor cells, currently used for chemosensitivity test should be accurately model in situ tumor. Thus we studied the merit and demerit of chemosensitivity testing using MTS.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kohno N: "Methotrexate, 5-Fluorouracil and leucovorin in patients with advanced head and neck cancer." J.Jpn.Soc.Cancer Ther.30. 556-560 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kohno N: "Salvage chemotherapy with PEM and Long CF regimen in CDDP refractory advanced head and neck carcinoma." Jpn.J.Cancer Chemother.22. 209-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 甲能 直幸: "頭頸部扁平上皮癌に対する化学療法" 癌と化学療法. 23. 277-282 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 甲能 直幸: "腫瘍塊(多細胞スフェロイド)を用いた感受性試験" 頭頸部腫瘍. 22. 488-495 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 楠 正恵: "頭頸部扁平上皮癌に対するシスプラチン、5フルオロウラシル療法-多細胞スフェロイドを用いた研究-" 順天堂医学. 42. 207-216 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 甲能 直幸: "頭頸部扁平上皮癌に対する化学療法の意義" JOHNS. 13. 1286-1292 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoyuki Kohno: "Induction chemotherapy with cisplatin, etoposide, and mitomycin-C (PEM) regimen in advanced cases with cancer of pharynx and oral cavity" Auris Nasus Larynx(Tokyo). 22. 49-52 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoyuki Kohno: "Salvage chemotherapy with Pem and long-CF regimen in CDDP refractory advanced head and neck cancer" Jpn J Cancer Chemother. 22. 209-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoyuki Kohno: "Methotrexate, 5-Fluorouracil and leucovorin in patients with advanced head and neck cancer." J.Jpn.Soc.Cancer Ther.30. 556-560 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoyuki Kohno: "Chemotherapy for the head and neck squamous carcinoma." Jpn J Cancer Chemother. 23. 277-282 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masae Kusunoki: "Cisplatin plus-5-fluorouracil therapy for the head and neck squamous carcinoma." Juntendo Igaku. 42. 207-216 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoyuki Kohno: "Drug response assay for the head and neck squamous carcinoma using multicellular tumor speroids" Head and neck Cancer. 22. 488-495 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naoyuki Kohno: "Effects of chemotherapy for the head and neck squamous carcinoma." JOHNS. 13. 1286-1292 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 楠 正恵: "頭頸部扁平上皮癌に対するシスプラチン、5フルオロウラシル療法-多細胞スフェロイドを用いた研究ー" 順天堂医学. 42. 207-216 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 甲能直幸: "頭頸部扁平上皮癌に対する化学療法の意義" JOHNS. 13. 1286-1292 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 甲能直幸: "腫瘍塊(多細胞スフェロイド)を用いた感受性試験)" 頭頚部腫瘍. 22. 488-495 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Naoyuki Kohno: "Induction chemotherapy with cisplatin, etoposide, and mitomycin-C (PEM) regimen in advanced cases with cancer of pharynx and oral cavity" Auris Nasus Larynx (Tokyo). 22. 49-52 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Naoyuki Kohno: "Salvage chemotherapy with Pem and long-CF regimen in CDDP refractory advanced head and neck cancer" Jpn J Cancer Chemother. 22. 209-214 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 甲能,直幸: "頭頸部扁平上皮癌に対する化学療法" 癌と化学療法. 23. (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 楠正恵: "頭頸部扁平上皮癌に対するシスプラチン、5フルオロウラシル療法-多細胞スフェロイドを用いた研究-" 順天堂医学. 42. (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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