• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Enhancement of antitumor activity of anticancer drugs and irradiation in head and neck cancer

Research Project

Project/Area Number 07671890
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKinki University School of Medicine

Principal Investigator

NISHIDA Shozo  Kinki University School, Medicine, Lecture, 医学部, 講師 (40208187)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Keywordsapoptosis / Mn-SOD / CuZn-SOD / SOD / Apoptosis / 頭頸部腫瘍
Research Abstract

It has been reported that radicals play some role in the induction of apoptosis by irradiation or TNFa. However, the body has scavengers of these radicals. In the present study, we attempted to investigate the effect of inhibition of these scavengers, particularly of cytoplasmic Cu, Zn-SOD that scavenge oxygen radicals, and the induction of apoptosis. Apoptosis was confirmed by the formation of an apoptosis body observed by light microscopy and fragmentation of DNA detected by agarose gel electrophoresis. Treatment of DDC caused a significant decrease in SOD activity. As the SOD activity decreased, the appearance of an apoptosis body increased in frequency. Pretreatment of NAC decreased induction of apoptosis by DDC.The above results suggested that elevation of intracytoplasmic oxygen radicals relative to decrease in the activity of Cu, Zn-SOD may lead to the induction of apoptosis.
Some malignant tumors are resistant to TNF-alpha or several antineoplastic agents which exert antitumoral effects mediated by the radicals. One predicted reason for this resistance is induction of an mitochondrial enzyme, Mn-SOD,which scavenges O_2 radicals by TNF-alpha. We investigated whether or not inhibition of this induction recovers the antitumoral effects of TNF-alpha. In the cell strain cultivated in a medium containing TNF-alpha, there were no dead cells. Addition of TNF-alpha significantly increased both the concentration and activity of Mn-SOD,which indicated the induction of Mn-SOD by TNF-alpha in the cell strain. This induction was inhibited by the addition of inhibitor. Further addition of TNF-alpha after the addition of inhibitor caused cell death in 50.3% of the cells.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 西田升三: "Cu,Zn-SOD阻害によるアポトーシスの誘導効果" 日本癌学会総会記事. 55. 305-305 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西田升三: "Mn-SOD阻害によるTNFαの壊死効果の増強" 日本癌学会総会記事. 53. 636-636 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shozo NISHIDA: "Induction of apoptosis by inhibition of Cu, Zn-superoxide dismutase in Hl60 cells" Proceedings of Japanese Cancer Association. 55. 305 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shozo NISHIDA: "Enhancement of TNF induced antitumor activity by inhibition of superoxide dismutase induction" Proceedings of Japanese Cancer Association. 53. 636 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西田升三: "Cu,Zn-SOD阻害によるアポトーシスの誘導効果" 日本癌学会総会記事. 55. 305-305 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西田升三: "Mn-SOD阻害によるTNFαの壊死効果の増強" 日本癌学総会記事. 55. 636-636 (1994)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi