| Project/Area Number |
07671895
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Aichi Cancer Center |
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Principal Investigator |
HASEGAWA Yasuhisa Aichi Cancer Center Research Institute, Research Fellow, 研究所, 研究員 (10261207)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMURA Tomohiro Okayama University School of Dental, Professor, 歯学部, 教授 (00028747)
MATSUURA Hidehiro Aichi Cancer Center Research Institute, Research Fellow, 研究所, 研究員 (60199750)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Chemosensitivity test / Head and Neck Cancer / Chemotherapy / HDRA / p53 / 頭頚部癌 / P53 / 制癌剤感受性試験 |
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| Research Abstract |
1. A study of an anticancer drug sensitivity test suing the SDI test : The succinate dehydrogenase inhibition (SDI) test was carried out for 55 head and neck cancers. As a results, the evaluable rate of the SDI test was 80%. Cancer cells from the involved lumph nodes showed a tendency to low calculated inhibition rate (i. R.) as compared with those from primary tumors. The in vitro results were compared with the clinical responses of the recurrent patients who were treated by CDDP/5FU.Predictive accuracies of CDDP and 5FU were 56%and 63%, respectively.
2. A study of an anticancer drug sensitivity test using the HSRA test : Histoculture drug response assay (HDRA) with MTT-endpoint was performed for 40 head and neck cancers. using a concentration-I.R.curve and a concentration-efficacy rate curve, the optimal cutoff concentration was estimated as 20mug/ml for CDDP,300mug/ml for 5FU and15mug/ for adriamycin. I.R.at CDDP 20mug/ml from the involved lumph nodes showed a significantly lower value than that of primary tumors of squamous cell carcinoma. The in vitro results at CDDP 20mug/ml were compared with the clinical responses of 13 patients who were treated by VDDP/5FU.Significant correlation between in vitro and in vivo results was observed. The predictive accuracy for response to chamotherapy was 77%.
3. Immunohistochamical study : The correlation betwen an anticancer sensitibity test and the status of p53, bcl-2, Glutathione S-Transferase pi and Metallothionein (MT) using immunohistochemical staining was examined. Positive staining of p53 and MT significantly correlated with lower I.R.at CDDP 20mug/ml of HDRA assay. Their predictive accuracy for response to chemotherapy was 75% and 50%, respectively.
4. Results : HDRA assay and the status of p53 using immonuhistochamical staining are extremely useful in the prediction of response to chamotheray for head and neck cancer.
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