| Project/Area Number |
07671918
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kobe University |
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Principal Investigator |
INOUE Masanori Kobe University, School of Medicine, Associate Professor, 医学部, 助教授 (90116187)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAHARA Yasutomo Kobe University, School of Medicine, Instructor, 医学部, 助手 (20236855)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | diabetes mellitus / visual pathway / GABA metabolism / optic nerve fiber / polyol metabolism / 網膜、上丘、大脳皮質 / 視路でのGABA代謝 / アルドース還元酵素・阻害剤 / 網膜内GABA |
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| Research Abstract |
Streptozotocin (STZ) was administrated to rats to induce diabetes. Loss of body weight and significantly elevated level of serum glucose were observed. After treatment of STZ,the GABA content in retina decreased compared to that of control animals. The glutamic acid decarboxylase (GAD) activity and the GABA transaminase (GABA-T) activity of diabetic group was decreased. The GABA content in the superior colluiculus (SC) and the cerebral cortex decreased compared to the control level. The GAD and GABA-T activity of SC and conrtex in DM animals also reduced. The GAD activity of diabetic group was markedly decreased. Decreases of GAB content were dependent on the inhibition of the GAD activity.
Structural impairments of optic nerve fibers in STZ-induced diabetic rat were investigated using morphometric analysis. Electron microscopic morphometry showed significantly smaller cross-sectional size of axons and myelin but no change of myelinated fiber number. Reductions of myelinated fiber size was especially remarkable in the larger fibers. ARI treatment improved structural abnormalities without any changes in body weight and blood glucose level. These findings suggest that structural impairment may contribute to the abnormalities of psychophysical and electrophysiological measurement detected in diabetes. Moreover, ARI treatment, which can improve the polyol metabolism, may have a beneficial effect on optic nerve impairment in diabetes.
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