| Project/Area Number |
07671924
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Wakayama Medical College |
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Principal Investigator |
OHNISHI Yoshitaka Wakayama Medical College, Department of Ophthalmology, Professor., 医学部・眼科, 教授 (80037473)
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| Co-Investigator(Kenkyū-buntansha) |
KAWANO Yoh-ichi Kyushu University, Faculty of Medicine, Department of Ophthalmology, Assistant P, 医学部・眼科, 講師 (90211185)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | elecrtrochemotherapy / electroporation / eyelid tumor / bleomycin / photodynamic therapy / hematoporphyrin derivative / photosensitizer |
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| Research Abstract |
The purpose of this study is to develop multidisciplinary treatments in ocular tumors, using electrochemotherapy, photodynamic therapy and hyperthermic treatment.
Malignant melanoma cells were cultured. Some of these sells were exposed to various intensities of electric pulse alone (500-2500)V/cm). Other cells were then exposed for 30 min to an antiproliferative agent : bleomycin, mitomycin C or 5-fluorouracil with or without electric pulse. Cell proliferation was assessed by cell counting on day 3 and by a H^3-thymidine uptake assay. A significant reduction in the cell number was observed only at 2500V/cm. Bleomycin, mitomycin C and 5-fluorouracil treatment inhibited cell proliferation in a dose-dependent manner either with or without electric pulse. Electric pulse treatment induced an inhibitory effect of bleomycin cell proliferation which was 100 times more prominent than bleomycin alone..
Malignant melanoma cells cultured were transplanted into the eyelid of the mouse. After the growth of the transplanted tumor cells, electrochemotherapy using electroporation method was performed. The drugs used were bleomycin, mitomycin C and 5-fluorauracil. The most effective drug is bleomycin for this electrochemotherapy.
The electrochemotherapy may therefore reduce the necessary dose of antiproliferative agents for the treatment against malignant tumors. We developed this electrochemotherapy for glaucoma surgery.
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