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Glycobiological Study of Malignant Tumors in Ophthalmology

Research Project

Project/Area Number 07671928
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKagoshima University

Principal Investigator

UEHARA Fumiyuki  Faculty of Medicine Kagoshima University Associate Professor, 医学部, 助教授 (30168653)

Co-Investigator(Kenkyū-buntansha) YANAGITA Toyoko  University Hospital Kagoshima University Research Associate, 医学部・附属病院, 助手 (40271142)
SAMESHIMA Munefumi  Faculty of Medicine Kagoshima University Research Associate, 医学部, 助手 (80041333)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsTumor / Glycobiology / Lectin / Sialyltransferase / Mucin / Ki 67 / Keratin
Research Abstract

The glycoconjugates in various malignant tumors in eyelids and orbita were analyzed by lectin histochemistry and in situ hybridization histochemistry using cRNA probes for sialiltransferases (ST). We considered that the cells, which expressed both cytoplasmic distribution of ST-mRNA and binding of lectins, specific for sialic acids, to the cell surfaces, were actively producing sialoglycans. We also considered that the cells, whose surfaces were stained with the lectins without cytoplasmic distribution of ST-mRNA,have completed the synthesis of sialoglycans. Based on these view points, this glycohistochemical technique is useful for dynamic analysis of the glycoconjugates in individual tumor cells. This technique revealed that O-linked sialoglycan, whose turnover-rate is slow, may be distributed over the cells of thickened spinocellular layr in the tumor of seborrheic keratosis and involved in its pathomechanism. We also analyzed the basal cell calcinoma in eyelids and the lacrimal pleomorphic adenoma using this method.
MUC 1, one of the O-linked glycoconjugates, was immunohistochemically detected in the tumor cells of all cases of squamous cell carcinoma and sebaceous carcinoma in eyelids whereas it was absent in those of all cases of basal cell carcinoma and benigh tumors in eyelids. These findings suggested that MUC 1 may be involved in expression of malignant features including metastasis. On the other hand, the Ki 67 antigen-expression rates of malignant eyelid tumors were higher than those of benigh tumor, suggesting that Ki 67 can be used to distinguish between malignant and benigh tumors. Immunohistochemical distribution of kearatin revealed that the basal cell carcinoma in eyelids is consisted of uniform tumor cells, all of which express the same keratin, while the squamous cell carcinoma and sebaceous carcinoma are composed of various differentiatiating tumor cells.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 上原文行、大庭紀雄: "眼科領域のリンパ系腫瘍の診断・分類法に関する1考察" あたらしい眼科. 12. 1489-1491 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上原文行、大庭紀雄: "眼窩リンパ系腫瘍のKi67抗原発現分布の免疫組織化学" あたらしい眼科. 13. 813-815 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 神村浩策、上原文行ほか: "反対側慢性上顎洞炎を随伴した涙腺多形腺腫の1例" あたらしい眼科. 13. 967-969 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上原文行、大庭紀雄: "眼瞼腫瘍のKi67抗原発現分布の免疫組織化学" あたらしい眼科. 13. 1411-1413 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 迫田由起子,上原文行ほか: "眼瞼腫瘍として発症した多形腺腫の1例" あたらしい眼科. 13. 1623-1625 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上原文行、柳田豊子ほか: "ラット眼瞼上皮の糖鎖組織化学的解析" 日本眼科学会誌. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uehara, F., Ohba, N.: "A new classification of lymphoid tumors in the orbital region" Atarashii Ganka (J.Eye.). 12-9. 1489-1491 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uehara, F., Ohba, N.: "Immunohistochemical analysis of Ki67 antigen in orbital lymphoid tumors" Atarashii Ganka (J.Eye.). 13-5. 813-815 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kamimura, K., Uehara, F., Ohba, N., Ito, K., Yoshida, H.: "A case of pleomorphic adenoma of the lacrimal gland with chronic maxillay sinusitis" Atarashii Ganka (J.Eye.). 13-6. 967-969 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uehara, F., Ohba, N.: "Immunohistochemical analysis of Ki67 antigen in eyelid tumors" Atarashii Ganka (J.Eye.). 13-9. 1141-1413 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakoda, Y., Uehara, F., Arimura, H., Ohba, N., Yoshida, H.: "A case of pleomorphic adenoma of the eyelid" Atarashii Ganka (J.Eye.). 13-10. 1623-1625 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Uehara, F., Yanagita, T., Iwakiri, N., Ohba, N.: "Glycohistochemical analysis of rat eyelid epithelium" Journal of Japanese Ophthalmol Society. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上原文行、大庭紀雄: "眼科領域のリンパ系腫瘍の診断・分類法に関する1考察" あたらしい眼科. 12. 1489-1491 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上原文行、大庭紀雄: "眼窩リンパ系腫瘍のKi67抗原発現分布の免疫組織化学" あたらしい眼科. 13. 813-815 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 神村浩策、上原文行ほか: "反対側慢性上顎洞炎を随伴した涙腺多形腺腫の1例" あたらしい眼科. 13. 967-969 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上原文行、大庭紀雄: "眼瞼腫瘍のKi67抗原発現分布の免疫組織化学" あたらしい眼科. 13. 1411-1413 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 迫田由紀子、上原文行ほか: "眼瞼腫瘍として発症した多形腺腫の1例" あたらしい眼科. 13. 1623-1625 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上原文行、柳田豊子ほか: "ラット眼瞼上皮の糖鎖組織化学的解析" 日本眼科学会誌. (印刷中).

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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