Examination of nucleic acid probes available for the experimental eye research

Research Project

Project/Area Number	07671942
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Ophthalmology
Research Institution	Kansai Medical University
Principal Investigator	YAMAGISHI Kazuya Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90174599)
Project Period (FY)	1995 – 1996
Project Status	Completed (Fiscal Year 1996)
Budget Amount *help	¥2,100,000 (Direct Cost: ¥2,100,000) Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords	Tachykinin receptor / Retrograde cell labeling / Optic nerve crush / Ganglion cell layr / Inner nuclear layr / 神経節細胞 / 単純ヘルペス / 眼内炎 / アンチセンスオリゴ核酸 / 遺伝子治療
Research Abstract	Substance P receptor is known to provide a principal interface between tachykinin peptides and tachykinin-sensitive cells in retinal circuitry and to produce several physiological functions such as excitation of ganglion cells. We reported results of in situ hybridization analysis of substance P receptor in rat retina using digoxigenin-labeled RNA probes to yield discrete cell labeling. Distinct hybridization signal was present in a great majority of ganglion cells that provide retinal fibers to a central target. It was also present in a subpopulation of amacrine cells. Following optic nerve crush, ganglion cells lost hybridization signal in a time-dependent manner, while hybridization-positive amacrine cells were persistently seen. From the results, we identified hybridization message distinctly localized to two systems, output cells and intrinsic cells in retinal circuitry.