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Examination of nucleic acid probes available for the experimental eye research

Research Project

Project/Area Number 07671942
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKansai Medical University

Principal Investigator

YAMAGISHI Kazuya  Kansai Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90174599)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsTachykinin receptor / Retrograde cell labeling / Optic nerve crush / Ganglion cell layr / Inner nuclear layr / 神経節細胞 / 単純ヘルペス / 眼内炎 / アンチセンスオリゴ核酸 / 遺伝子治療
Research Abstract

Substance P receptor is known to provide a principal interface between tachykinin peptides and tachykinin-sensitive cells in retinal circuitry and to produce several physiological functions such as excitation of ganglion cells. We reported results of in situ hybridization analysis of substance P receptor in rat retina using digoxigenin-labeled RNA probes to yield discrete cell labeling. Distinct hybridization signal was present in a great majority of ganglion cells that provide retinal fibers to a central target. It was also present in a subpopulation of amacrine cells. Following optic nerve crush, ganglion cells lost hybridization signal in a time-dependent manner, while hybridization-positive amacrine cells were persistently seen. From the results, we identified hybridization message distinctly localized to two systems, output cells and intrinsic cells in retinal circuitry.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Kondoh,A.: "In situ hybridization analysis of substance P receptor in the rat retina." Experimental Brain Research. 112. 181-186 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akitoshi Kondoh, Takeshi Houtani, Teizo Ueyama, Kazuyasu Baba, Michiko Ikeda, Kazuya Yamagishi, Hirohiko Miki, Masanobu Uyama, Shigetada Nakanishi and Tetuo Sugimoto: "In situ hybridization analysis of substance P receptor in the rat retina." Experimental Brain Research. 112 (2). 181-186 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kondoh,A: "In situ hybridization analysis of substance P receptor in the rat retina" Experimental Brain Reseach. 112. 181-186 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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