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Ultrastructural studies of the multinucleation of osteoclasts ; the role and function of mononuclear osteoclasts.

Research Project

Project/Area Number 07671959
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

DOMON Takanori  Hokkaido Univ., Sch.of Dent., instructor, 歯学部, 助手 (50217618)

Co-Investigator(Kenkyū-buntansha) IIZUKA Tadashi  Hokkaido Univ., Sch.of Dent., instructor, 歯学部, 助手 (80168062)
TAKAHASHI Shigeru  Hokkaido Univ., Sch.of Dent., instructor, 歯学部, 助手 (70241338)
YAMAMOTO Tsuneyuki  Hokkaido Univ., Sch.of Dent., assosiate prof., 歯学部, 助教授 (80200822)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsodontoclast / osteoclast / distribution of number of nuclei / mononuclear cell / multinucleation / 連続切片 / 単核
Research Abstract

Osteoclasts and odontoclasts have been considered multinucleated giant cells which resorb hard tissue by ruffled borders. Recently, the authors reported the presence of a nomonuclear osteoclast and odontoclast with a ruffled border. However, the relative frequency of such cells and the distribution of the number of nuclei including mononuclear cells in them have not been elucidated.
Six human deciduous teeth were used in this study. After fixation and decalcification, tartrate-resistant acid phosphatase (TRAP) activity was detected with the azo dye method, and then TRAP-positive cells were observed on resorbing areas of teeth by light microscopy. The cells for investigation were serially sectioned by semithin sections to observe the presence of resorptive lacuna and the number of nuclei.
The TRAP activity was detected in both multinucleated and mononuclear odontoclasts from serial semithin sections, and 242 TRAP-positive cells which formed lacunae on dentin were investigated to determinie the frequency distribution of the number of nuclei. The mean number of nuclei per cell was 5.3, and median was 4. Only 2.9 per cent of odontoclasts were mononucleus and 93.8 per cent had 10 or fewer nuclei.
The majority of odontoclasts forming lacunae on the dentin were cells with 10 or ferwer nuclei, and mononuclear odontoclasts parcipitated in human deciduous tooth resorption together with multinucleated ones.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] T. Domon: "Mononuclean adontoclast parcipitation in tooth vesorption." The Anatomical Record. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 土門卓文: "in vivoならびにin vitroにおける単核の破骨細胞の三次元構造;準超薄切片と超薄切片の連続薄切法を用いた三次元立体複構" 日本電子顕微鏡学会第40回シンポジウム論文集. 75-78 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.DOMON: "Mononuclear Odontoclast Parcipitation in Tooth Resorption ; The Distribution of Nuclei in Human Odontoclasts" Anat.Rec.submitted. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Domon: "Mononuclear odontoclast parcipitation in tooth resorption" The Anatomical Record. (発表予定).

    • Related Report
      1996 Annual Research Report
  • [Publications] 土門卓文: "in vivoならびにin vitroにおける単核の破骨細胞の三次元構造;準超薄切片と超薄切片の連続薄切法を用いた三次元立体複構" 日本電子顕微鏡学会 第40回シンポジウム論文集. 75-78 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 長内正数 他: "ヒト乳歯歯根吸収面に存在する破歯細胞の微細形態学的研究" 小児歯科学雑誌. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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