• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

INVOLVEMENT OF NITRIC OXIDE IN MAINTAINING AND FACILITATING THE HYPERALGESIA ASSOCIATED WITH CHRONIC NOCICEPTION IN THE SITE OF THE FIRST SYNAPTIC RELAY OF THE PAIN PATHWAY

Research Project

Project/Area Number 07672013
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

YONEHARA Norifumi  Osaka University Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (70124534)

Co-Investigator(Kenkyū-buntansha) TAKEMURA Motohide  Osaka University Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (70192169)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsNO / NADPH-diaphorase / nociception / dorsal horn / N^<omega>-L-arginine methyl ester / N^ω-nitro-L-arginine methyl ester / アジュバンド / 下歯槽神経 / 三叉神経感覚核
Research Abstract

In order to elucidate the involvement of nitric oxide in spinal nociceptive processing, a correlation of thermal withdrawal latency with nitric oxide synthase-stained neurons in the rat lumbar dorsal horn was analyzed after adjuvant- or heat-induced inflammation. From 4 hrs through 5 days after subcutaneous injection of complete Freund's adjuvant into the hind paw, a marked thermal hyperalgesia was observed following heat stimulus applied to the affected region. In control rats, NADPH-diaphorase-positive neurons were observed in laminae I through V with a higher density at the border between laminae II and III in the lumbar spinal dorsal horn, and they were also stained immunohistochemically for rat cerebellar nitric oxide synthase. NADPH-diaphorase- and nitric oxide synthase-positive neurons increased significantly in the superficial layrs of the dorsal horn ipsilateral to the inflamend hind paw at day 3 of adjuvant-induced inflammation. No change in NADPH-diaphorase-positive neurons was observed at 1 hr and 1 day of adjuvant-induced inflammation, or at 1 hr, 1 and 3 days of heat-induced inflammation (47゚C for 30 min). The intravenous administration of N^<omega>-L-arginine methyl ester (LNAME,50 mg/kg), an antagonist of nitric oxide synthase, significantly blocked the adjuvant-induced thermal hyperalgesia at day 3 of inflammation, but not at day 1, and had no effect in non-inflamed rats. This anti-hyperalgesic effect of LNAME at day 3 of inflammation was reversed by the priodministration of L-arginine (500 mg/kg i.p.), a substrate of nitric oxide synthase.
These data suggest that nitric oxide producing neurons in the site of the first synaptic relay of the pain pathway are involved in maintaining and facilitating the hyperalgesia associated with chronic nociception.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] N. Yonehara, et al: "Contribution of bradykinin to heat-induced substance P release in the hind instep of rats" Life Sei.56. 1679-1688 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J. Takagi, et. al.: "A possible involvement of monoaminergic and opioidergic systems in the analgesia induced by electro-acupuncture" Japan, J, Phanmacol.70. 73-80 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Yonehara, et al.: "Involvement of calcium-activated potassium channels in inhibitory prejunctional effect of morphine on peripheral sensory nerves" Regulatory Peptides. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Yonehara: "Involvement of nitric oxide in re-innervation of rat molar tooth pulp following transection of the infeiror alveolar nerve" Brain Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 米原 典史: "神経ペプチド" 炎症と抗炎症戦略,医薬ジャーナル. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Yonehara, K.Saito, S.Oh-ishi, M.Katori and R.Inoki: "Contribution of bradykinin to heat-induced substance P release in the hind instep of rats" Life Sci. 56. 1679-1688 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Takagi, T.Sawada and N.Yonehara: "A possible involvement of monoaminergic and opioidergic systems in the analgesia induced by electroacupuncture" Japan.J.Pharmacol.70. 73-80 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Yonehara and S.Takiuchi: "Involvement of calcium-activated potassium channels in inhibitory prejunctional effect of morphine on peripheral sensory nerves" Regulatory Peptides. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Yonehara: "Involvement of nitric oxide in re-innervation of rat molar tooth pulp following transection of the infeiror alveolar nerve" Brain Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Yonehara.et.al.: "Contribution of bradykinin to heat-induced substance P release in the hind instep of rats" Life Science. 56. 1679-1688 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.Takagi.et.al: "A possible involvement of monoaminegic and opiordergic systems in the analgesia induced by Electroacupuncture in rabbits" Japan.J.Pharmacol. 70. 73-80 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Yonehara,et.al: "Involvement of calcium-activated potassum channels in inhibitory prejundional effect of morphine on peripheral sensory nerues" Regulatory Peptides. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Yonehara: "Involvement of nitric oxide in re-innervation of rot molar tooth pulp following transection of the infeiror alveolar nerve" Brain Res.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 米原典史: "神経ペプチド" 炎症と抗炎症戦略.医薬ジャーナル. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 谷口恭章: "ケトプロフェン含有パップ剤(KPP)の外用鎮痛作用" 薬理と治療. 23. 2233-2238 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.Yonehara: "Contribution of bradykinin to heat-induced substance P release in the hind instep of rats" Life Science. 56. 1679-1688 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.Takagi: "A possible involvement of monoaminergic and opioidergic systems in the analgesia induced by electre-acupuneture" Japan.J.Pharmacol.70. 73-80 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Takemura: "NADPH-diaphorase in the developing rat : Lower brainstem and cervical spinal cord with special reference to the trigemino-solitary complex" J.Comp.Neuro.365. 511-525 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi