| Project/Area Number |
07672023
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Health Sciences University of Hokkaido, School of Dentistry |
|---|
Principal Investigator |
TOJYO Yosuke Health Sciences University of Hokkaido, School of Dentistry, Associate Professor, 歯学部, 助教授 (90111731)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANIMURA Akihiko Health Sciences University of Hokkaido, School of Dentistry, Assistant Professor, 歯学部, 講師 (70217149)
|
|---|
| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | Parotid cells / Intracellular Ca^<2+> / Capacitative Ca^<2+> entry / Intracellular Ca^<2+> stores / Kinase inhibitor / Phosphatase inhibitors / Purinergic receptor / カルシウム流入 / 細胞内カルシウム動態 / カルシウムチャンネル / プロテインキナーゼ / プロテイン・ホスファターゼ |
|---|
| Research Abstract |
1) Effects of the protein kinase inhibitor staurosporine on capacitative Ca^<2+> entry in rat parotid cells. Staurosporine significantly enhanced Ca^<2+> entry from extracellular medium activated by depletion of intracellular Ca^<2+> stores.
2) Effects of serine/threonine phosphatase inhibitors on capacitative Ca^<2+> entry in rat parotid cells. The phosphatase inhibitors (calyculin-A, tautomycin, okadaic acid) suppressed the Ca^<2+> entry induced by depletion of intracellular Ca^<2+> stores.
These results suggest that the capacitative Ca^<2+> entry may be regulated by protein phosphorylation/dephosphorylation.
3) ATP-induced Ca^<2+> entry in rat parotid cells. Ectracellular ATP increased cytosolic Ca^<2+> concentration without formation of inositol phosphates. This increase appears to be due to an entry of Ca^<2+> via activation of P_<2Z> purinoceptors.
4) Imaging of intracellular Ca^<2+> mobilizing in rat parotid acinar cells. When parotid acinus was stimulated with carbachol, the initial rise in cytosolic Ca^<2+> was detected in the apical pole of the cells. Thereafter, the increase spreaded into the basolateral area. When Ca^<2+> was added to the medium following depletion of intracellular Ca^<2+> stores, an increase in cytosolic Ca^<2+> was observed in the basal pole.
5) Visualization of intracellular Ca^<2+> stores in a human parotid ductal cell line. The intracellular Ca^<2+> stores were visualized using digital imaging techniques with a confocal laser microscopy. The results demonstrate that the entire ER and the nuclear envelop serve as IP_3-sensitive Ca^<2+> stores.
|
