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Effect of CFS-1 on osteoclast and its precursor cells

Research Project

Project/Area Number 07672028
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

YAMADA Shoji  Showa University, School of Dentistry, Professor, 歯学部, 教授 (00111617)

Co-Investigator(Kenkyū-buntansha) SUZUKI Keiko  Showa University, School of Dentistry, Iecturer, 歯学部, 講師 (50119187)
AMANO Hitoshi  Showa University, School of Dentistry, Iecturer, 歯学部, 講師 (90212571)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCSF-1 / osteoclast / fusion / osteopontin / actin-ring / NHEl / intracellular Ca^<2+> / tyrosine kinase / 細胞内Ca^<2+> / Osteopontin / in situ hybridization / 接着因子 / カルシトニン / 酒石酸抵抗性酸性フォスファターゼ / マクロファージ / Wortmannin / Herbimycin A
Research Abstract

Colony-stimulating factor-1 (CSF-1), Also called macrophage colony-stimulating factor, is required for growth, differentiation activation, and survival of cells of the mononuclear phagocytic system. This cytokine has been shown to be essential foe osteoclast development as well as for inducing both proliferation and differentiation of osteoclast progenitors. It also sustains survival of mature osteoclasts and stimulates spreading and migration of these cells. In the present in vitro study, the formation of large TRAP-positive cells with a high number of nuclei was observed when osteoclasts isolated from rat long bones were incubated with CSF-1. These large cells, cultured on plastic, bind calcitonin and form F-actin along the edges of the cells. Fusion to such large TRAP-positive multinucleated cells in the presence of CSF-1 and the formation of pits were also observed on dentine slices. Quantitative data obtained from cultures on plastic demonstrated that the number of osteoclasts slightly increased in the course of 72 hrs in the presence of 250 pM CSF-1, whereas it decreased rapidly after 24 hrs in the absence of CSF-1, which confirms that this cytokine is required for the survivai of osteoclasts. The number of nuclei per osteoclast was maximal after 16 hrs incubation with CSF-1, namely twice the value found in the absence of CSF-1. The maximal effect of the cytokine on the fusion process was observed at a concentration of 250 pM-500pM.A calculation of the medians of the average frequency of nuclei distribution per osteoclast resulted in 4 nuclei per osteoclast in the absence, and 6 in the presence of CSF-1. Genistein and herbimycin A,inhibitors of tyrosine kinases, inhibited the fusion induced by CSF-1. The data suggest that CSF-1 induces osteoclast fusion, and that tyrosine kinase (s) are involved in this process. The fusion process may continue throughout the entire life on an osteoclast.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Amano H. Yamada S. and Felix R: "Colony-stimulating factor-1 (CSF-1) stimulates the fusion process in osteoclasts." J. Bone Miner. Res.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Teramoto T: "The effect of extracellular pH on the adherence and differentiation of isolated rat osteoclasts." Jpn. J. Oral Biol.40(1). 62-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 天野 均: "破骨細胞による骨吸収のメカニズム" 歯科医療. 11. 22-28 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amano H. Yokose S. Ikeda T. et al.: "Colony-stimulating factor-1 (CSF-1) induces osteopontin mRNA expression in cultured rat osteoclasts." Dentistry in Japan. 33. 37-39 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Jimi E. Nakamura I. Amano H. et al.: "Osteoclast function is activated by osteoblastic cells through a mechanism involving cell-to cell contact." Endocrinology. 137. 2187-2190 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amano H. Hofstetter W. Cecchini M. et al.: "Downregulation of colony-stimulating factor-1 (CSF-1) binding by CSF-1 in isolated osteoclasts." Calcif. Tissue Int.57. 367-370 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Amano: "fos gene and bone disease." The Bone.Vo1.9. 45-50 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Amano, W Hofstetter, M Cecchini, H Fleisch, R Felix: "Downregulation of colony-stimulating factor-1 (CSF-1) binding by CSF-1 in isolated osteoclasts." Calcif.Tissue Int.Vo1.57. 367-370 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E Jimi, I Nakamura, H Amano, Y Taguchi, T Tsurukai, M Tamura, N Takahashi, T Suda: "Osteoclast function is activated by osteoblastic cells through a mechanism involving cell-to-cell contact." Endocrinology. Vol.137. 2187-2190 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Amano, S Yokose, T Ikeda, K Suzuki, T Teramoto, S Yoshiki, S Yamada: "Colony-atimulating factor-1 (CSF-1) induces osteopontin mRNA expression in cultured rat osteoclasts." Dentistry in Japan. Vol.33. 37-39 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Amano: "Regulation of bone resorption by osteoclast." ShikaIryo.Vol.11. 22-28 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H Amano, S Yamada, R Felix: "Colony-stimulating factor-1 (CSF-1) stimulates the fusion process in osteoclasts." J.Bone Miner.Res.Vol.13(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T Teramoto: "The effect of extracellular pH on the adherence and differentiation of isolated rat osteoclasts." Jpn.J.Oral Biol.Vol.40. 62-72 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amano H.Yamada S.and Felix R: "Colony-stimulating factor-1 (CSF-1) stimulates the fusion process in osteoclasts." J.Bone Miner.Res.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 天野 均: "破骨細胞による骨吸収のメカニズム" 歯科医療. 11. 22-28 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Amano H.Yokose S.Ikeda T.et al.: "Colony stimulating factor-1 (CSF-1) induces osteopontin mRNA expression in cultured rat osteoclasts." Dentistry in Japan. 33. 37-39 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 天野均: "破骨細胞による骨吸収のメカニズム" 歯科医療. 11(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Amano,S.Yokose,T.Ikeda et al.: "Colony stimulating factor-1(CSF-1) induces psteopontin mRNA expressio in cultured rat osteoclasts." Dentistry in Japan. 33(in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] E.Jimi,I.Nakamura,H.Amano et al.: "Osteoclast function is activated by osteoblastic cells through a mechanism involving cell-to cell contact." Endocrinology. 137. 2187-2190 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Suzuki and S.Yamada: "Humoral factor of ascites sarcoma 180 stimulate osteoblastic UMR 106-01 cell proliferation and bone resorption via signal transduction pathways which are clealy different from clearly different from those of parathyrpid hormone." Jpn.J.Pharmacol.72. 149-159 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Amano H.: "Downregulation of colny-stimulating factor-1 (CSF-1) binding by CSF-1 in isolated osteoclasts." Calc. Tiss. Int.57. 367-370 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Morohashi T.: "Effects of Strontium on Calcium Metabolism in Rats. II. Strontium Prevents the Increased Rate of Bone Tuurover in Ovariectomized Tats." Jpn. J. Pharmacol.68. 153-159 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Horohasi t.: "Hypoclcemia following strontium injection is associated with serum parathyroid hormone and calcitonin levels in rats." Jpn. J. Oral Biol.37. 332-334 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Skai N.: "Biological Mechanisms of Tooth Movement & Craniofacial Adaptation" edited by Davidovitch, Z., EBSCCOMedia, Birmingham, Alabama, USA (in press),

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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