| Project/Area Number |
07672035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
病態科学系歯学(含放射線系歯学)
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
SHIBATA Kenichiro Hokkaido Univ.Sch.Dent., Associate Prof., 歯学部, 助教授 (50145265)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Tsuguo Hokkaido Univ.Sch.Dent., Prof., 歯学部, 教授 (10064362)
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| Project Period (FY) |
1995 – 1996
|
| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | mycoplasma / phospholipase C / T cells / apoptosis / TNF-alpha / fibroblast / IL-6 / IL-8 / TNFα / 口腔マイコプラズマ / コンカナバリンA |
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| Research Abstract |
1. AIDS-associated mycoplasmas possessed phosphatidylcholine- and phosphatidylinositol (PI) -specific phospholipases C (PLC) in the membrane. PLC are shown to be a virulence determinant of bacteria. Especially, PI-PLC are known to release GPI-anchored proteins such as CD14, CD55 and Fc receptor which play important roles in the defense system of the host.
2. Oral and AIDS-associated mycoplasmas possessed the activity to enhance concanavalin A-induced apoptosis of mouse splenic T cells. TNF-alpha was found to play an key role in the expression of the activity.
3. Oral mycoplasmas induces interleukin-6 and interleukin-8 in human gingival fibroblasts by a mechanism different from that of lipopolysaccharides of gram-negative bacteria.
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