• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of the Mechanical Loading on the Proliferation and Differentiation of Osteoblast-Derived Culture Cell.

Research Project

Project/Area Number 07672105
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 補綴理工系歯学
Research InstitutionTokyo Medical and Dental University

Principal Investigator

HAMAZAKI Tatsuo  Tokyo Medical and Dental University, Dentistry, Research Associate, 歯学部, 助手 (70228534)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsmouse / osteoblasts / mechanical load / clinorotation / cell cycle / gene expression / cell growth / 遺伝子発現 / 骨芽細胞 / 増殖率 / クリノスタット
Research Abstract

Mechanical load play an important role on the bone growth during the formation and remodeling of the whole bone including scaletal and mandiblar bones. To clarify the effects of mechanical load on the cells growth and formation of bone, we analyzed the reactivity of osteoblast-derived cells, MC3T3-El cells, under some different kind of mechanical loads. One is gravitational vector-averaged clinorotation (GVC) which was considered to be canceled out the gravity effect on cultured cells as well as plants and animal embryos by diffusing the load. Another is centrifugation culture by which continuous high gravity was loaded on the cultured cells.
We already reported that the proliferation rate of osteoblast-derived MC3T3-E1 cells was suppressed when they cultured under the GVC.The suppression rate was maximally over 65% compared to the control cells. In the present study, we performed a flow cytometric analysis using aphidicolin-treated synchronous cultured cells. As the results, it was rev … More ealed that the cell cycle was suppressed at the S phase progression and also showed a delay on the transfers from G_1 phase to S phase. On the other hand, the proliferation rate was increased when the MC3T3-E1 cells were cultured under the mechanical load produced by the centrifugation. The rate of increments of cell growth cultured under hyper gravity after 48 hr was about 13% at 30 G and about 26% at 60 G,respectively. In this case, the S phase progression was fastened about 10% compared to the control. Moreover, some of cell growth-related gene expressions were changed when they cultured under the different mechanical loads. The c-fos and c-myc expressions were transitory increased when the cell was cultured under the mechanical load. On the other hand, c-fos expression was decreased under the GVC.No difference was observed on the expression of c-jun and p53 as well as the control genes, G3PDH and beta-actin. It is important to make a series of the studies on the effect of mechanical load to the cultured osteoblasts and to be considered that it will lead us to be clarify the relationship between the mechanical load and the bone formation and remodeling. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Yamagami,K.: "Intrahepatic Formation of SF Substance, the Precursors of Egg Envelope Proteins, in Response to Estrogen Administration in Fish." Perspectives Comparative Endocrinology. 94. 316-324 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Duke,P.J.: "Clinorotation Inhibits Chondrogenesis in Micromass Cultures of Embryonic Mouse Limb Cells." Environmental Medicine. 39. 1-12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 浜崎 辰夫: "骨芽細胞の増殖関連遺伝子発現に及ぼす荷重の影響" 口腔病学会雑誌. 63. 524- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 新井 泉: "システムダイナミクスモデルを応用した細胞周期の動的解析" 歯科材料・器械. 15. 459-466 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 佐藤 温重: "微小重力下での培養へのアプローチ" 組織培養. 22. 381-384 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamagami, K., Murata, K., Hamazaki, T.S., and Iuchi, I.: "Intrahepatic Formation of SF Substances, the Precursors of Egg Envelope Proteins, in Response to Estrogen Administration in Fish." Perspectives Comp.Endocrinol.94. 316-324 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Duke, J., Sato, A., Hamazaki, T., and Montufar-solis, D.: "Clinorotation Inhibits Chondrogenesis in Micromass Cultures of Embryonic Mouse Limb Cells." Environment.Med.39. 1-12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamazaki, T., and Sato, A.: "Suppression of the proliferation of osteoblast cultured under the clinorotation." Space Util.Res.12. 24-27 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamazaki, T., and Sato, A.: "Effect of gravitational changes on the cell cycle of cultured osteoblast." Sci.Space Biol.9. 196-197 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arai, I., Masuda, I., Hamazaki, T., Sato, K., and Sato, A.: "The Dynamic Analysis of Cell Cycles with the System Dynamics Model." J.J.Dent.Mater.15. 459-466 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sato, A., Hamazaki, T., Masuda, I., Hongo, T., and Sato, K.: "An approach of Tissue and Cell Culture under Microgravity" Tissue Culture. 22. 381-384 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamazaki, T.and Sato, A.: "Effects of Mechanical Load on the Cell Growth-Related Gene Expression of Osteoblast in Culture." J.Stomatol.Sci., Jpn.63. 524 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamazaki, T., Ohmura, T., Sato, K., Hongo, T., and Sato, A.: "Effects of Mechanical Loading on the Gene Expressions of Osteoblast Cells." J.J.Dent.Mater.15 (27S). 138-139 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamazaki, T., and Sato, A.: "Effects of Hypergravity on the Cell Proliferation and Gene Expressions of Osteoblast Cells." Space Util.Res.13. 5-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arai, I., Masuda, I., Hamazaki, T., Sato, K., and Sato, A.: "The dynamic model analysis of cell cycles with the system dynamics." J.J.Dent.Mater.15 (S28). 132-133 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamagami,Murata,Hamazaki,and Iuchi: "Intrahepatic Formation of SF Substances,the Precursors of Egg Envelope Proteins,in Response to Estrogen Administration in Fish." Perspectives Comparative Endocrinology. 94. 316-324 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Duke,Sato,Hamazaki,and Montufar-solis: "Clinorotation Inhibits Chondrogenesis in Micromass Cultures of Embryonic Mouse Limb Cells." Environmental Medicine. 39. 1-12 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 浜崎辰夫・佐藤温重: "骨芽細胞の増殖関連遺伝子発現に及ぼす荷重の影響" 口腔病学会雑誌. 63. 524- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 新井、増田、浜崎、佐藤、佐藤: "システムダイナミクスモデルを応用した細胞周期の動的解析." 歯科材料・器械. 15. 459-466 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐藤、浜崎、増田、本郷、佐藤: "微小重力下での培養へのアプローチ" 組織培養. 22. 381-384 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jackie Duke: "Clinorotation Inhibits Chondrogenesis in Micromass Cultures of Embryonic Mouse Limb Cells." Environmental Medicine. 39. 1-12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浜崎辰夫: "培養細胞の細胞周期に及ぼす重力変化の影響" 宇宙生物科学. 9. 196-197 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浜崎辰夫: "クリノスタット回転培養下における骨芽細胞の増殖抑制" 第12回宇宙利用シンポジウム. 24-27 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi