| Project/Area Number |
07672150
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
NISHIMURA Taiichi ASAHIKAWA MEDICAL COLLEGE,DEPARTMENT OF ORAL & MAXILLOFACIAL SURGERY,INSTRUCTOR, 医学部, 講師 (30113749)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEKAWA Masanori ASAHIKAWA MEDICAL COLLEGE,DEPARTMENT OF ORAL & MAXILLOFACIAL SURGERY,ASSISTANT, 医学部, 助手 (50216876)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | BMP-2 / immunohistochemistry / periosteal cells / induce bone formation / autoradiography / tritiated thymidine / 遊離骨膜移植 |
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| Research Abstract |
1. Immunohistochemistry
(1) In Free Grafts 5 days postgrafting, bone formation was found and the positive BMP-2 stained cells were mesenchymal cells, osteoblasts and osteocytes. BMP-2 was not detected in the muscle and mesenchymal cells around the muscle.
(2) BMP-2 was not detected in the matrices of bone and cartilage.
(3) At 14 days postgrafting, Osteoclast-like cells stained positively.
2. The formation of the bone formed by grafts isolated within millipore diffusion chambers.
(1) Within the millipore diffusion chambers 14 days postgraftings, the periosteal cells had differentiated to form cartilage and osteochondroid. During the 28 day course of the study, we never observed cartilage and bone formation at the millipore diffusion chamber-host tissue interface.
3. Autoradiography
(1) The autoradiographs of pre-graft periosteal tissue showed that 5.2% of the graft cells were labeled with radiothymidine (^3Htdr).
(2) There were ^3Htdr-labeled mesenchymal cells which surrounded the newly formed bone as well as a few labeled osteocytes.
In summary, these results suggest that while periosteal cambial cells express BMP-2, the amount of BMP-2 produced by periosteal cells may be insufficient to induce bone formation from the host mesenchymal cells and that the ossicles were formed by osteoblasts derived from the cambial cells in the periosteal grafts.
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