| Project/Area Number |
07672164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | School of Medicine, Kanazawa University |
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Principal Investigator |
NAKAGAWA Kiyomasa School of Medicine, Kanazawa University, Assosiated Professor, 医学部・歯科口腔外科学講座, 助教授 (30155676)
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| Co-Investigator(Kenkyū-buntansha) |
OKABE Koichi School of Medicine, Kanazawa University, Clinical Instructor, 医学部・歯科口腔外科学講座, 講師 (70242518)
TAKATSUKA Shigeyuki School of Medicine, Kanazawa University, Clinical Instructor, 医学部・歯科口腔外科学講座, 講師 (00251926)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Odontogenic infection / murine abscess model / microbial interaction / Streptococcus constellatus / Fusobacterium nucleatum / oxdation-reduction potential (Eh) / 歯性感染症 / Streptococcus constel1atus / 口腔感染症 / 混合感染 / 好気性菌 / 嫌気性菌 / 発生機序 / 病原性 / 酸化還元電位 |
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| Research Abstract |
In our previous clinical study, it appeared mixed infection of Steptococcus and anaerobic gram-negative rods was detected in severe odontogenic infections. Thus, we determined the characteristics of microbial interaction of Steptococcus constellatus and Fusobacterium nucleatum, which were the prime pathogens of orofacial odontogenic infection. In the study using murine abscess model, the microbial synergism between S.constellatus and F.nucleatum was found. In this synergism, the product of S.constellatus was not able to enhance F.nucleatum virulence. In contrast, it appeared the heat-stable substance in the product of F.nucleatum could enhance the virulence of S.constellatus. Moreover, it appeared the modulation of host cells function caused by F.nucleatum was one of mechanisms of this microbial synergism. We considered the oxdation-reduction potential (Eh) as the role of enhancement of F.nucleatum virulence caused by S.constellatus. Our in vitro study indicated that the occurrence of mixed infection of the oral region was closely related to the Eh and that the pathogenicity of bacteria was dependent largely on the combination of Viridans streptococci and anaerobes.
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