Effect of site selective cAMP & its metabolite on human adenocarucinoma cell line

• Project/Area Number: 07672177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: University of Tokushima
• Principal Investigator: YOSHIDA Hideo University of Tokushima, School of Dentistry, Assosiate Professor, 歯学部, 助教授 (30116131)
• Project Period (FY): 1995 – 1996
• Project Status: Completed (Fiscal Year 1996)
• Budget Amount: ¥2,200,000 (Direct Cost: ¥2,200,000); Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000); Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 8-chloro-aenosine 3' : 5'-monophosphate (8-Cl-cAMP) / 8-chloro-adensine / human adenocarcinoma cell line / cAMP-dependent protein kinase (PKA) / cell proliferation

Research Abstract

We examined the effects of 8-chloro-adenosine 3' : 5'-monophosphate (8-Cl-cAMP), site-selective analogue of cAMP,and its metabolite 8-chloro-adenosine (8-Cl-ad) on the proliferation of human adenocarcinoma cell line HSG cells and its derivative cell line HSG-AZA1, HSG-AZA3, and human adenoid squamou carcinoma cell line TYS cells in culture. Cultivation of these cells in thepresence of 8-Cl-cAMP (0.5muM) or 8-Cl-ad (lmuM) resulted in the growth inhibition and reduction of colony formation rates on plastic surface or in soft agar to under 10%. Whereas the morphological alteration of treated cells were not shown. Then, it was confirmed that the HSG cells treated with 8-Cl-cAMP (0.5muM) or 8-Cl-ad (1muM) were not induced apoptosis, because DNA pattern was similar to untreated cells in agar gel electrophoresis. In addition, the cAMP-dependent protein kinase A,isolated from treated HSG cells, were decreased.

Research Products

• [Publications] Sato M,Harada K,Bando T,Shirakami T,Nakashiro K,Yoshida H,et al: "Characteristics of antitumor activity of 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinone)against a human adenoid squamous carcinoma-forming cell line grown in athymic nude mice." Cancer Letters.91(1),. 1-9 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sato M,Iga H,Yoshioka N and Yoshida H.: "Induction of differentiation into osteoblasts by treatment with 22-oxa-la,25-dihydroxyvitamin D3 in human salivary cancer cells." Differentiation Therapy.Challenges of Modern Medicine.10,. 329-333 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sato M,Iga H,Yoshioka N,Fukui K,Kawamata H,Yoshida H,Hirota S and Kitamura Y: "Emergence of osteoblast-like cells in a neoplastic human salivary cancer cell line after treatment with 22-Oxa-la,25- dihydroxyvitamin D_3." Cancer Lett.(in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sato M,Kawamata H,Harada K,Nakashiro K,Ikeda Y,Gohda H,Yoshida H,et al: "Induction of cyclin-dependent kinase inhibitor, p21^<KAF:> by treatment with 3,4-dihydro-6-[4-(3,4)-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinon) in a human salivary cancer cell line with mutant p53 gene." Cancer Lett.(in press). (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sato M,Harada K,Bando T,Shirakami T,Nakashiro K,Yoshida H,Nakai S,Kawai K and Adachi M: "Characteristics of antitumor activity of 3,4-dihydro-6-[4- (3,4-dimethoxybenzoyl) -1-piperazinyl]-2 (1H)-quinolinone (vesnarinone) against a human adenoid squamous carcinoma-forming cell line grown in athymic nude mice" Cancer Letters. 91(1). 1-9 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sato M,Iga H,Yoshioka N and Yoshida H: "Induction of differentiation into osteoblasts by treatment with 22-oxa-la, 25-dihydroxyvitamin D3 in human salivary cancer cells" Differentiation Therapy. Challenges of Modern Medicine. 10. 329-333 (1995)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sato M,Iga H,Yoshioka N,Fukui K,Kawamata H,Yoshida H,Hirota S and Kitamura Y: "Emergence of osteoblast-like cells in a neoplastic human salivary cancer cell line after treatment with 22-Oxa-la, 25-dihydroxyvitamin D" Cancer Lett.(in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sato M,Kawamata H,Harada K,Nakashiro K,IKeda Y,Gohda H,Yoshida H,Nishida T,Ono K,Kinoshita M and Adachi M: "Induction of cyclin-dependent kinase inhibitor, p21^<WAP1>, by treatment with 3,4-dihydro-6-[4-(3,4)-dimethoxybenzoyl)-1-piperazinyl]-2 (1H)-quinolinone (vesnarinon) in a human salivary cancer cell line with mutant p53 gene" Cancer Lett. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sato M,Harada K,Bando T,Shirakami T,Nakashiro K,Yoshida H,et al: "Characteristics of antitumor activity of 3,4-dihydro-6-[4-(3,4-dimethoxy-benzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinone)against a human adenoid squamous carcinoma-forming cell line grown in athymic nude mice." Cancer Letters.91(1). 1-9 (1995)
• [Publications] Sato M,Iga H,Yoshioka N and Yoshida H:"Induction of differentiation into osteoblasts by treatment with 22-oxa-1a,25-dihydroxyvitamin D3 in human salivary cancer cells." Differentiation Therapy.Challenges of Modern Medicine.10. 329-333 (1995)
• [Publications] Sato M,Iga H,Yoshioka N,Fukui K,Kawamata H,Yoshida H,Hirota S and Kitamura Y: "Emergence of osteoblast-like cells in a neoplastic human salivary cancer cell line after treatment with 22-Oxa-1a,25-dihydroxyvitamin D_3." Cancer Lett.(in press). (1997)
• [Publications] Sato M,Kawamata H,Harada K,Nakashiro K,Ikeda Y,Gohda H,Yoshida H,et al: "Induction of cyclin-dependent kinase inhibitor,p21 by treatment with 3,4-dihydro-6-[4-(3,4)-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone(vesnarinon)in a human salivary cancer cell line with mutant p53 gene." Cancer Lett.(in press). (1997)