| Project/Area Number |
07672200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | School of Dentistry, Aichi-Gakuin University |
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Principal Investigator |
YOSHIDA Kenji First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-Gakuin University.Associate prof., 歯学部・口腔外科学第1講座, 助教授 (40183701)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Michio First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-G, 歯学部・口腔外科学第1講座, 講師 (20261026)
KATO Mugio First Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichi-G, 歯学部・口腔外科学第1講座, 講師 (30214406)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Low Power Laser / Cultured Fibroblast Cells / Cell Proliferation / TIMP-1 / TIMP-2 |
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| Research Abstract |
The present study showed that the biological effects on the cultured human gingival cells irradiated by two different wave length low-power lasers. Ana a control was set up in the non-irradiation group.
Results : Mitogen activity was increased by both wave length laser irradiations when the He-Ne watt density was 18.72 mW/cm^2, and the joule density was 5.62 J/cm^2, and when the Ga/Al/As diode watt density was 1.12 mWcm^2, and the joule density was 0.33 J/cm^2. The cell proliferation was increased under a 10% TIMP-1 and TIMP-2 free FCS+D-MEM condition. 48 hours after He-Ne irradiation and 24 hours after Ga-Al-As diode irradiation, there were significant differences. At that time, when TIMP-1 and TIMP-2 secretions in condition medium were measured with EIA,TIMP-1 secretion had significantly increased after 24 hours from irradiation with both lasers together with the He-Ne laser group and Ga-Al As diode laser group. But TIMP-2 detection was impossible by EIA.In the same culture conditions, when the Ga-Al-As diode laser was used to irradiate Gin-1 cells and the RT-PCR method was used to detect mRNA expression, an obvious difference in the TIMP-1 mRNA expression was found at 2 hours post-irradiation. But no difference in TIMP-2 mRNA expression was noted.
These results suggest that low-power lasers of appropriate irradiation accelerate human gingival fibroblast cell growth, and may promote acceleration of wound healing.
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