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Cytobiological Studies of Fibroblasts Derived from Human Deciduous Periodontal Ligament. -Regulation of Proliferation and Differentiation by Retinoic Acids-

Research Project

Project/Area Number 07672243
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionKanagawa Dental College

Principal Investigator

UCHIMURA Noboru (1996)  Kanagawa Dental College, School of Dentistry, Professor, 歯学部, 教授 (80104454)

大西 武 (1995)  神奈川歯科大学, 歯学部, 助手 (50213799)

Co-Investigator(Kenkyū-buntansha) KIMOTO Shigenari  Kanagawa Dental College, School of Dentistry, Assistant, 歯学部, 助手 (90205013)
KUMASAKA Sumio  Kanagawa Dental College, School of Dentistry, Lecturer, 歯学部, 講師 (10161697)
KAWASE Toshio  Kanagawa Dental College, School of Dentistry, Professor, 歯学部, 教授 (30084784)
SAITO Shigeru  Kanagawa Dental College, School of Dentistry, Professor, 歯学部, 教授 (80084713)
内村 登  神奈川歯科大学, 歯学部, 教授 (80104454)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsperiodontal ligament of human deciduous teeth / cultured cell / retinoic acid / vitamin D_3 / cell proliferation / cell differentiation / 乳歯 / 歯根膜 / ビタミンA / retinoic acid / all-trans retinoic acid / 9-cis retinoic acid
Research Abstract

Retinoic acids (RA), which are natural derivatives of vitamin A,have a wide spectrum of biological roles in growth and differentiation of tissues. We have demonstrated the effects of retinoic acids of all-trans retinoic acid and 9-cis retinoic acid on the cell growth, alkaline phosphatase (ALPase) activity and protein synthesis of human deciduous and permanent periodontal ligments derived fibroblasts (HPLF-Y and HPFL,respectively). Ceels migrated from the explanted human periodontal ligaments were cultured in 5% FCS D-MEN without ascorbic acid and exposed with all-trans RA or 9-cis RA.All-trans RA and 9-cis RA inhibited the cell growth. The ALPase activity of HPLF-Y was markedly stimulated by all-trans RA and 9-cis RA at the concentration of 10nM and 1nM,respectively, ALPase activity of HPLF was stimulated by all-trans RA and 9-cis RA at the concentration of 10nM,1,25 (OH)_2D_3 altered the cell growth and differentiation of HPLF-Y and HPLF exposed with all-trans RA or 9-cis RA for 8 da … More ys. In the growth phase, HPLF-Y did not alter the incorporation of ^3H-thymidine (TdR) into cold TCA precipitates of cell layrs, but the DNA synthesis of HPLF was inhibited by all-trans RA and 9-cis RA.In the pre-confluent and confluent phase, all-trans RA and 9-cis RA decreased the incorporation of ^3H-TdR in the cold TCA precipitates of HPLF-Y and HPLF.The total incorporation of ^<35>S-methionine (Met) was markedly decreased by the addition of all-trans RA,9-cis RA and D_3, The extracellular proteins labeled with ^<35>S-Met were separated into three fractions by IEC-DEAE column eluted with 0 to 0.5M NaCl in 20mM Tris-HCl, pH 8.0 which were non interacted, 0.1M NaCl and 0.2M NaCl eluted fractions. The 0.2M NaCl eluted fraction containing osteonectin and 0.1M NaCl eluted fraction were decreased by all-trans RA and 9-cis RA.On the other hand, non-interacted fraction was increased by D_3 and all-trans RA,Therefore, all-trans RA and 9-cis RA may modulate the cell differentiation of periodontal ligament cells. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kawase T.,Saito s.: "Osteoblastic character of fibroblasts derived from human periodontal Iigament." Tissue Culture Research communication. 14. 109-125 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishiyama K,Nozaki N,Kawase T and Saito S.: "Effect of Mechanical Suress on Protein Synthesis in Human Periodontal Ligament Cells." Bull of Kanagawa Dent Col. 23・1. 1-8 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki Y.,Kasai Y.,Satoyoshi M.,Suzuki Y.,Kawase T.,and Saito S.: "Matrix mineralization and the differentiation of osteocyte-like cells in culture." Journal of Bone and Mineral Research. 10. 231-242 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoyoshi M.,Koizumi T.,Teranaka T.,Iwamoto T.,Saito S.and Mikuni-Takagaki Y.: "Extracellular processing of dentin matrix protein in the mineralizing odontoblast culture." Calcified Tissue International. 57. 237-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 大西武,木本茂成,川瀬俊夫: "ヒト乳歯歯根膜由来線維芽細胞の細胞生物学的研究:レチノイン酸による増殖と分化に対する調節" 神奈川歯学. 31・1. 1-18 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mokuni-Takagaki Y.,Suzuki Y.,Kawase T.,and Saito S.: "Distinct responses of different populations of bone cells to mechanical stress." Endocrinology. 137. 2028-2035 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 川瀬俊夫,出口眞二,斎藤茂(下野正基,飯島国好編): "治癒の病理<臨床編>第3巻 歯の移植・再植-歯根膜を生かす-2歯根膜の特性,2-2歯根膜細胞の特性." 医歯薬出版, 219 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawase T.and Saito S.: "Osteoblastic character of fibroblasts derived from human periodontal ligament." Tissue Culture Research Communication. 14. 109-125 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishiyama K., Nozaki N., Kawase T.and Saito S.: "Effect of Mechanical Stress on Protein Synthesis in Human Periodontal Ligament Cells." Bull of Kanagawa Dent Col.23 (1). 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki Y., Kasai Y., Satoyoshi M., Suzuki Y., Kawase T.and Saito S.: "Matrix Mineralization and the Differentiation of Osteocyte-like Cells in Culture." Journal of Bone and Mineral Research. 10. 231-242 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoyoshi M., Koizumi.T., Teranaka T., Iwamoto T., Saito S.and Mikuni-Takagaki Y.: "Extracellular Processing of Dentin Matrix Protein in the Mineralizing Odontoblast Culture" Calcified Tissue International. 57. 237-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Onishi T., Kimoto, S.and Kawase T.: "Cytobiological Studies of Fibroblasts Derived from Human Deciduous Periodontal Ligament.-Regulation of Proliferation and Differentiation by Retinoic Acids-" Kanagawashigaku. 31 (1). 1-18 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki Y., Suzuki Y., Kawase T.and Saito S.: "Distinct Responses of Different Populations of Bone Cells to Mechanical Stress." Endocrinology. 137. 2028-2035 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawase T.,Saito S.: "Osteoblastic character of fibroblasts derived from human periodontal ligament." Tissue Culture Research Communication. 14. 109-125 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishiyama K,Nozaki N,Kawase T and Saito S.: "Effect of Mechanical Stress on Protein Synthesis in Human Periodontal Ligament Cells." Bull of Kanagawa Dent Col. 23・1. 1-8 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mikuni-Takagaki Y.,Kasai Y.,Satoyoshi M.,Suzuki Y.,Kawase T.,and Saito S.: "Matrix mineralization and the differentiation of osteocyte-like cells in culture." Journal of Bone and Mineral Research. 10. 231-242 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Satoyoshi M.,Koizumi T.,Teranaka T.,Iwamoto T.,Saito S.and Mikuni-Takagaki Y.: "Extracellular processing of dentin matrix protein in the mineralizing odontoblast culture." Calcified Tissue International. 57. 237-241 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大西武,木本茂成,川瀬俊夫: "ヒト乳歯歯根膜由来線維芽細胞の細胞生物学的研究:レチノイン酸による増殖と分化に対する調節" 神奈川歯学. 31・1. 1-18 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mikuni-Takagaki Y.,Suzuki Y.,Kawase T.,and Saito S.: "Distinct responses of different populations of bone cells to mechanical stress." Endocrinology. 137. 2028-2035 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 川瀬俊夫,出口眞二,斎藤滋(下野正基,飯島国好編): "治癒の病理<臨床編>第3巻 歯の移植・再植-歯根膜を生かす-2歯根膜の特性,2-2歯根膜細胞の特性." 医歯薬出版, 219 (1995)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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