• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Search for Antitumor Principles from Medicinal Plants by Monitoring Nitric Oxide Production in Activated Macrophages

Research Project

Project/Area Number 07672254
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical pharmacy
Research InstitutionTohoku University

Principal Investigator

FUSHIYA Shinji  Tohoku University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (80108563)

Co-Investigator(Kenkyū-buntansha) TAKANO Fumihide  Tohoku University, Faculty of Pharmaceutical Sciences, Research Assistant, 薬学部, 教務職員 (20236251)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsmacrophage / nitric oxide / antitumor activity / anti-inflammarory activity / Acer nikoense / Veronicastrum sibiricum / Menispermum dauricum / Sinomenium acutum / マクロファージ / 冬虫夏草 / 免疫増強作用 / オパイン
Research Abstract

Search for medicinal plant constituents active toward nitric oxide production in activated macrophages was carried out. The result of this investigation is as follows :
1) Nitric oxide production in activated macrophages was suppressed by AcOEt soluble portion of the methanol extract of Acer nikoense, and (+)-rhododendrol was isolated as an active compound. On the other hand, the n-BuOH soluble portion increased the nitric oxide production, and polar polyphenols were assumed to be active substances. Epi-rhododendrin isolated from the n-BuOH soluble portion, depressed the nitric oxide production.
2) The methanol extract of Veronicastrum sibiricum increased the nitric oxide prodiction, and the activity passed into the AcOEt solible portion. Picroside I was isolated as an active compound from the AcOEt soluble portion with a new inactive compound, 6-(3,4-dimethoxy-cinnamoyl) ajugol.
3) Both methanol extracts of Menispermum dauricum and Sinomenium acutum depressed the nitric oxide production. Dauricine and sinomenine were isolated as active compounds, respectively.
4) The culture dluid of Isaria japonica enhanced the production of anti-SRBC plaque forming cells (PFC) in mice. It also recovered the reduction of anti-SRBC PFC response and number of spleen cells caused by treatment with 5-fluorouracil.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Shinji Fushiya: "New Opine Type Amino Acids frem a Peisonous Mushroom,Clitocybe Acremelalga" Tetrahedren. 52. 877-886 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fumihide Takano: "The Culture Fluid of Isaria Japenice Yasuda Arguments Anti-SRBC Antubedy Response in Mice" Biological and Phamarceutical Bulletin. 19. 641-643 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinji Fushiya, Mamoru Matsuda, Shohei Yamada and Shigeo Nozoe: "New Opine Type Amino Acids from a Poisonous Mushroom, Clitocybe Acromelalga" Tetrahedron. 52. 877-886 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fumihede Takano, Yumi Kikuchi, Shinji Fushiya, Hiroshi Hojo, Shigeo Nozoe, Nobuo Yahagi, Remiko Yahagi and Yoshikazu Kondo: "The Culture Fluid of Isaria Japonica Yasuda Arguments Anti-SRBC Antivody Response in Mice" Biological and Pharmaceutica Bulletin. 19. 641-643 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinji Fushiya: "New Opine Type Amine Acids from a Poisoneus Mushrosom, Clitocybe Acromelalga" Tetrahedron. 52. 877-886 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fumihide Takano: "The Culture Fluid of Isaria Japonica Yasuda Arguments Anti-SRBC Antibody Response in Mice" Biological and Pharmaceutical Bulletin. 19. 641-643 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shinji Fushiya: "New Opine Type Amino Acids from a Poisonsus Mushroom, Clitocybe Acremelalga" Tetrahedron. 52. 877-886 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fumihide Takano: "The Culture fluid of Isaria Japonica Yasuda Areguments Anti-SRBC Antibody Response in Mice" Biological and Pharmaceutical Bulletin. 19. (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi