• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of New Preparation Method of Artificial Membrane Vaccine Using Spontaneous Transfer of Membrane Protein

Research Project

Project/Area Number 07672311
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Physical pharmacy
Research InstitutionTOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY

Principal Investigator

UENO Masaharu  Toyama Medical and Pharmaceutical University, Dept of Pharmaceutical Sci., Professor, 薬学部, 教授 (40080197)

Co-Investigator(Kenkyū-buntansha) HAYASHI Kyoko  Toyama Medical and Pharmaceutical University, Dept. of Medicine, Research Associ, 医学部, 助手 (60110623)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsartificial membrane vaccine / membrane protein / influenza virus / liposomes / protein transfer / zeta potential / DMPC / membrane fluidity / 人口膜ワクチン / CVI細胞 / SUV / ζポテンシャル / 表面構造 / DMPC / 膜蛋白質
Research Abstract

Spontaneous transfer of protein from either influenza virus-infected or uninfected cells to dimyristoylphosphatidylcholine (DMPC) liposomes was examined. The amount of transferred protein of liposomes incubated with influenza virus-infected cells (I-lipo) was more than that of liposomes incubated with uninfected cells (U-lipo). The ratio of the amount of spontaneous transfer protein of I-lipo to that of U-lipo increased in proportion to the diameter of liposomes except for small unilamellar vesicles. The amounts of transferred protein of negative-charged liposomes composed of DMPC and stearic acid or dicetylphosohate were larger than that of neutral DMPC liposomes. There was a correlation between zeta potential of liposomes and the contents of transferred protein. Membrane fluidity, however, was appeared not to be responsible for the enhancing effect of negative charged liposomes on spontaneous protein transfer.
Therefore, we concluded that membrane surface structure based on electrostatic condition of liposomes will play an important role in the spontaneous protein transfer from cell membrane to recipient vesicles.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K. Kogure, M. Itoh, C. Nakamura K. Hayasi, M. Ueno: "Spoutaneous Transter of Vival Protein from Membrane of Influenza Vivus-Intected Cells to Liposomes in Dependent on the Pianeter of Receiver" Biol. Pharm. Bull.18 (8). 1168-1170 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小暮健太朗,上野雅晴,寺田 弘: "DDS技術としてのリポソーム研究の進歩" Fragrance J. 24. 66-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Kogure, O. Okuda T. Itoh K. Hayashi M. Ueno: "Development of Membraue Fusible Drug Cavrier from Erythroevte by the Spontanous Transtev of Uiral Fusion Protein from Inflneuza Uivas-Intected cells" Biol. Pharm. Bull.19 (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Kogure, M. Itoh, M. Nakamura, K. Hayashi, M. Ueno: "Progress in Drug Derivery System U ed by s. Hirota" Biomedical Research Founclution (Tokyo), 182 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kogure, M.Itoh, K.Hayashi and M.Ueno: "Spontaneous Transfer of Viral Protein from Membrane of Influenza Virus-Infected Cells to Liposomes is Dependent on the Diameter of Receiver" Biol. Pharm. Bull.18 (8). 1168-1170 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kogure, M.Itoh, C.Nakamura, K.Hayashi and M.Ueno: "Effect of Memmbrane Surface Structure on Spontaneous Transfer of Protein from Membrane of Influenza Virus-Infected Cells to Liposomes" Progress in Drug Delivery System V, ed by S.Hirota, p71-74, Biomedical Research Foundation, Tokyo. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kogure, M.Ueno and H.Terada: "Advanced Research on Liposome for Drug Delivery System" Fragrance J.24. 66-72 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kogure, C.Nakamura, O.Okuda, K.Hayashi and M.Ueno: "Development of Membrane Fusible Drug Carrier from Erythrocyte by the Spontaneous Transfer of Viral Fusion Protein from Influenza Virus-Infected Cells" Biol. Pharm. Bull.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小暮健太朗,上野雅晴,寺田弘: "DDS技術としてのリポソーム研究の進歩" Fragrance J.24. 66-72 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Kogure. O.Okuda,T.IToh,K.Hayashi,M.Ueno: "Deuelopement of Membrane Fusible Drng Carrier from Erythroyte by the Spondaneous Transfer of Viral Fusion Protein from Influenza Virus-Infected cells" Biol.Pharm.Bull.,. 19(in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Kogure. M.Itoh,C.Nakamura.K.Hayashi,M.Ueno: "Progress in Drug Derivery system V ed by S.Hirota" Biomedical Research Foundation (Tokyo), 182 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Kogure,M.Itoh,U.Kayashi,M.Ueno: "Spontaneous Transter of Viral Protein from Membrane of Intluenza Virus-Intecfed Cells to Liposomes is Dependent on the Diameter of Receiver" Biol. Pharm. Bull.18. 1168-1170 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi