| Project/Area Number |
07672337
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YOKOI Tsuyoshi Hokkaido Univ., Fac.of Pharm.Res.Associate Prof., 薬学部, 助教授 (70135226)
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| Co-Investigator(Kenkyū-buntansha) |
KAMATAKI Tetsuya Hokkaido Univ., Fac.of Pharm.Res.Prof., 薬学部, 教授 (00009177)
NAKAYAMA Katsuo Hokkaido Univ., Fac.of Pharm.Res.Instructor, 薬学部, 助手 (20261323)
藤田 健一 北海道大学, 薬学部, 教務職員 (60281820)
佐久間 勉 北海道大学, 薬学部, 助手 (30250468)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | genotyping / SM-12502 / coumarin / poor metabolizer / polymorphism / CYP2A6 / population study / チトクロームP450 2D6 / チトクロームP450 2A6 / drugmetabolism / 発がん / 代謝的活性化 / CYP2D6 / poor metabolizer / CYP1A2 |
|---|
| Research Abstract |
Genetic polymorphism of CYP2A6 was investigates. (+) -cis-3,5-dimethyl-2- (3-pyridyl) thiazolidin-4-one hydrochloride (SM-12502) was found to be a specific substrate of CYP2A6. Genomic DNA form extensive metabolizers and poor metabolizers (PM) of SM-12502 were examined by Southern blot analysis with CYP2A6cDNA probe. A new Sac I-RELP which distinguish PM was investigated. Finally, we concluded that the PM of SM-12502 was responsible for CYP2A6 whole gene deletion. A new genotyping method for CYP2A6 whole gene deletion with PCR was developed.
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