• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genes those regulate internal osmolality

Research Project

Project/Area Number 07672344
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionChiba University

Principal Investigator

NAKAMURA Tatsunosuke  Chiba University・Faculty of Pharmaceutical Science・Research Associate, 薬学部, 助手 (20114308)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordstrkA / mscL / nhaB / osmolality / gene / proC
Research Abstract

(1) trkAv and neighboring genes.
We clone K^+ uptake genes, trkAv and trkHv, from marine bacterium, Vibrio alginolyticus, those functionally complement K^+ uptake deficient E.coli strains. K^+ uptake activities of those strains with trkAv and/or trkHv genes are determined. Expression of TrkAv and TrkHv were examined also.
(2) mscL gene.
We examined the activity of MscL in Vibrio alginolyticus.
(3) nhaBv gene.
We cloned nhaBv, the second Na^+/H^+ antiporter, gene from V.alginolyticus. Topological experiments were done by phoA gene fusion method. It was appeared that NhaBv has odd number transmembrane segments.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Tatsunosuke Nakamura et al.: "Cloning and seqnencing of novelgenes from Vibrio alginolyticns" Biochemica et Biophysica Acta. 1277. 201-208 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tatsunosuke Nakamura et al.: "Cloning and seqnencing of the nhaB gene encoding an Na^+/H^+ antipester." Biochemica et Biophysica Acta. 1275. 157-160 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tatsunoske Nakamura et al.: "Cloning and sequencing of novel genes from Vibrio alginolyticus" Biochemica et Biophysica Acta.vol 1277. 201-208 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tatsunosuke Nakamura et al.: "Cloning and sequencing of the nhaB gene encoding an Na^+/H^+ antiporter." Biochemica et Biophysica Acta.vol 1275. 201-208 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tatsunosuke Nakamura et al.: "Cloning and sequencing of novel genes from Vibrio alginolyticus" Biochimica et Biophysica Acta. 1277. 201-208 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatsunosuke Nakamura et al.: "Cloning and sequencing of the nhaB gene encoding an Na^+/H^+ antiporter" Biochimaca et Biophysica Acta. 1275. 157-160 (1996)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi