| Project/Area Number |
07672376
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
NAKAGAWA Yasuhito Kitasato Univ.Pharmaceutical Sciences Professor, 薬学部, 教授 (00119603)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | oxidative stress / lipid peroxide / oxidative phospholipid / aldehydic phospholipid / ageing / 生成膜 / 生体膜 |
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| Research Abstract |
The aim of this project was to examine the metabolism of oxidized phospholipids in biological membranes peroxidized with different kinds of radicals which were alkyl radical produced with AAPH and hydroxy radical with xanthin/xanthin oxidase. CGP hydroperoxide as a primary product of peroxidation significantly formed in RBC membrane peroxidized with AAPH.No consisitent generation of CGP hydroperoxide was evident in XO/XOD/Fe peroxidation, indicating that CGP hydroperoxide is instable in this system. Aldehydic CGP as a secondary product of hydroperoxide decomposition also detected in AAPH-peroxidized RBC membrane. AAPH mainly oxidized CGP to hydroperoxide and aldehydic phospholipids and the production of thiobarbityric acid-reactive substance (TBARS) was relatively low. On the other hand, XO/XOD/Fe peroxidation caused the potential decompositon of phospholipids to eliminate large amounts of TBARS in conrast to generate a trace amounts of oxidized phopholipids. The present study indicates that the process of peroxidation of phospholipids in biomembrane greatly depends on their strucure and the peroxidation condition.
The amounts of oxidized phospholipids were also determined in various tissues of young and old rats. Phospholipid hydroperoxides and aldehydic phospholipids in liver, brain heart and kidney of old rats were significantly higher than those of young rats.
The present studt demonstrated the productions of oxidazed phospholipids, especially aldehydic phospholipids in biological samples suffered with oxidative stress at the first time.
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