• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Mechanism for Redox-Regulation of the JE gene

Research Project

Project/Area Number 07672382
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionShowa University

Principal Investigator

NOSE Kiyoshi  Professor, Showa University School of Pharmaceutical Sciences, 薬学部, 教授 (70012747)

Co-Investigator(Kenkyū-buntansha) MASHIMO Jun'ichi  Assistant professor, Showa University School of Pharmaceutical Sciences, 薬学部, 助手 (60054045)
EGAWA Kiyoshi  Lecture, Showa University School of Pharmaceutical Science, 薬学部, 講師 (00095879)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsactive oxygen / JE gene / NFkappaB / transcription / レドクス / 転写制御
Research Abstract

The JE gene encodes a secreted protein that participates in inflammation and immunological responses. Its expression is induced by various growth factors and stimuli. We previously showed that the induction of JE by phorbol ester was inhibited by scavengers of oxygens, and active oxygens seemed to be involved in the signal transduction of JE gene expression. In the present study, we focused to identify transcriptional regulatory region of the JE gen, especially active oxygen-responsive element in 5'-upstream region. Using CAT as a reporter, various deletions of 5'-upstream region of rat JE gene were constructed, and transcriptional response was measured by transfection into Balb 3T3 cells. About 400 bp element that locates between-1600 to-1200 bp upstream was found to participate in transcriptional activation through active oxygens, and this element contained well known NFkappaB sites. Gel shift assay using the NFkappaB as a probe, activation of binding activity of Balb 3T3 unclear extract by phorobol ester was inhibited in the presence of radical scavengers. The above results indicates that active oxygens produced from cells stimulated with phorbol ester have positive effect on the activation of NFkappaB that is most important in the transcriptional activation of JE gene.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Nose,k & Chiba,M.: "Functional activation of the efel gene by hyolrogen poloxide" Biochem,J. 316. 381-383 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Patel,Cannol,Amter,Nose & Cooke: "Cloning and ealy clofsal axial expression of flik." Develop.Biology. 178. 327-342 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arata.Hamaguchu & Nose: "Inhibition of NIHCells by the expression of the HSP27" J.Cell.Physiol.170. 19-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nose, K., and Ohba, M: "Functional activation of the egr-1 (early growth response-1) gene by hydrogen perxide" Biochem.J. 316. 381-383 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Patel, K., Connoly, D.J., Amthor, H., Nose, K., and Cooke, J: "Cloning and early dorsal axial expression of Flik, a chiken follistatin-related gene : Evidence for involvement in dorsalization. neural induction" Developmental Biology. 178. 327-342 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arata, S., Hamaguchi, S., and Nose, K: "Inhibition of NIH3T3 cells by the expression of the small molecular wight heat shock protein HSP27 : Involvement of its phosphorylation and aggregation at the C-terminal region" J.Cell.Physiol. 170. 19-26 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibanuma.M., Mochizuki, E., Maniwa, R., Mashimo, J., Nishiya, N., Imai, J., Takano, T., Oshimura, M., and Nose, K: "Induction of senescencelike phonotypes by the forced expression of hic-5 encoding a novel LIM motif protein in immortalized human fibroblasts" Mol.Cell.Biol. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Murata, M., Arata, S., and Nose, K: "Involvement of reactive oxygen species in the induction of chemokine JE/MCP-1 gene by phorbol-12-myristate-13 acetate in Balb 3T3 cells" Cell Struct.& Funtion. (in press). (1977)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mashino, J., Maniwa, R., Sugino, H., and Nose, K.Decrease: "the expression of a novel TGF bl-inducible and ras-recision gene, TSC-36 , in human cancer cells" Cancer Lett. (in press). (1977)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] NOSE,K.& Ohba,M.: "Functional activation of the egr-1 gene by bydregen peroxide" Biochem.J.316. 381-383 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Patel,Camoly,Amthol,Nose a caroke20GB02:Cloning and ealy dorsnl axial expression of Fhk.: Develop. Biology. 178. 327-342 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arate,Hamaquler a Nose: "Inhibition of Niheills by the expression of the HSP27" J.Cin.Physiol.170. 19-26 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arata, S., Haraguchi, S. & Nose K.: "Effect of the overexpression of HSP27 on the sensitivity of human fibroblast cells exposed to oxidative stress" J. Cell. Physiol.163. 458-465 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shibanuma, M. et al.: "Activation of DNA synthesis and expression of the JE gene by catalase in mouse osteoblastic cells." Exp. Cell Res.218. 132-136 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Egawa, K., et al.: "Isolation of a novel ras-recision gene that is induced by hydrogen peroxide" Febs Lett.372. 74-77 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi