| Project/Area Number |
07672383
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Showa University |
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Principal Investigator |
MIURA Nanko Showa University, School of Pharmaceutical Sciences, Assistant Professor, 薬学部, 助手 (10146904)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | IHRP / ITI / Inflammation / Acute-phase protein / Dextran sulfate / Heart ischemia-reperfusion / PK-120 / ビクニン / コンドロイチン硫酸 / ヒアルロン酸 / カリクレイン |
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| Research Abstract |
I discovered a novel glycoprotein from the eluate of the dextran sulfate column of plasmapheresis for the treatment of hypercholesteremia patients. Because its amino acide sequence was similar to those of the heavy chains of ITI family, I named its IHRP (ITL family heavy chain-related protein). The heavy chains of ITI family contained the amino acid sequence of DPHFII which is necessary for the complex formation with bikunin via chondroitin sulfate. IHRP lacks this sequence and it does not from a complex with bikunin. The IHRP gene located on chromosome 3p21-p14, on which the genes of heavy chain 1 and 3 of ITI family also exsted. Porcine IHRP mRNA was induced like acute-phase protein in the liver after the heart ischemia and reperfusion. Human IHRP gene expanded in about 15 kb and consisted of 24 exons. The gene had no TATA box and similar structure to that of ITI heavy chain gene. There were LF-A1, HNF-5, NF-IL6 and C/EBP on the promotor region. The gene was located at the 3'down-stream of EST gene. Mouse IHRP was induced in the inflammation after the treatment with turpentine or LPS.The induction with turpentine was blocked with dexamethason but it with LPS was not. The gel-shift assay with mouse IHRP gene promotor and the nuclear extract from the mouse liver after the turpentine treatment showed the clear gel-shift, however, the extract from the normal liver did not.
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