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Studies on mechanisms of development of airway hyperresponsiveness in rat bronchial asthma

Research Project

Project/Area Number 07672392
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionHoshi University

Principal Investigator

MISAWA Miwa  Hoshi University School of Pharmacy, professor, 薬学部, 教授 (20061294)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsbronchial asthma / airway hyperresponsiveness / muscarinic receptors / G protein / Ca^<2+> channel / neurokinins / beta-receptors / 気道過敏性 / 気管支平滑筋 / sensory neuropeptide / ACh受容体 / neutral endopeptidase / Ca availability
Research Abstract

The mechanisms of airway hyperresponsiveness (AHR) which lays behind bronchial asthma were investigated with rat allergy-induced AHR model developed by us. The following results were obtained.
1. Affinity of high affinity agonist binding sites of muscarinic receptor on bronchus was remarkably enhanced in AHR rats. The high affinity seemed to be linked to an increase in G protein level.
2. The bronchial constriction induced by cumulative administration of Ca^<2+> when preincubated with ACh under Ca^<2+>-free condition was significantly greater in AHR bronchus than in normal one. This suggests that availability of Ca^<2+> that liberates from sarcoplasmic reticulum and/or influxes into cells through receptor-operated Ca^<2+> channel is augmented in AHR.
3. The neutral endopeptidase (NEP) activity of AHR bronchus was decreased, and NEP inhibitors themselves enhanced the ACh-induced bronchial constriction. It is therefore suggested that neurokinins accumulation in bronchial tissues evokes an incrased ACh release from cholinergic nerve endings through NK_2 receptors which leadsto AHR.
4. Taking consideration of the results on receptor binding assays, the function and number of beta-receptors in bronchial tissues are unaltered in AHR,which denies the involvement of down-regulation of beta-receptors in the development of AHR.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Chiba,Y.& Misawa,M.: "Characteristic of muscarinic cholinoceptors in airways of antigen-induced airway hyperresponsiveness" Comp.Biochem.Physiol.111C. 351-357 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba,Y.& Misawa,M.: "Alteration in Ca^<2+> availavility involved in rat antigenically-induced airway hyperresponsiveness" Eur.J.Pharmacol. 278. 79-82 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba,Y.& Misawa,M.: "Inhibition of neutral endopeptidase increases airway responsiveness to Ach in nonsensitized normal rats" J.Appl.Physiol.78. 394-402 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba,Y.& Misawa,M.: "Characteristics of β-adrenoceptors in airways of antigen-induced airway hyperresponsive rats" Pathophysiology. 3. 157-161 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Misawa,M,Sato,J.,et al.: "Abnomal modulation of cholinegic neurotransmission by opioid in hyperresponsive bronchus of rats" Gen.pharmacol. 27. 441-444 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Misawa,M.& Sato,J.: "Abnormal modulation of cholinergic neurotransmission by endogenous nitric oxide in the bronchus of rats with AHR" Jpn.J.Pharmacol. 73. 125-132 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba,Y.& Misawa,M.,et al.: "Elevated nitric oxide synthase activity at antigen-induced airway hyperresponsiveness in rats" Am.J.Respir.Cell Mol.Biol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba, Y.and Misawa, M.: "Characteristic of muscarinic cholinoceptors in airways of antigen-induced airway hyperresponsiveness" Comp.Biochem.Physiol.111C. 351-357 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba, Y.and Misawa, M.: "Alteration in Ca^<2+> availability involved in rat antigenically-induced airway hyperresponsiveness" Eur.J.Pharmacol.278. 79-82 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba, Y.and Misawa, M.: "Inhibition of neutral endopeptidase increases airway responsiveness to ACh in nonsensitized normal rats" J.Appl.Physiol.78. 394-402 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba, Y.and Misawa, M.: "Characteristic of beta-adrenoceptors in airways of antigen-induced airway hyperresponsive rats" Pathophysiology. 157-161 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Misawa, M., Sato, J., Furukawa, Y., Chiba, Y.and Hosokawa, T.: "Abnormal modulation of cholinergic neurotransmission by opioid in hyperresponsive bronchus of rats" Gen. Pharmacol.27. 441-444 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Misawa, M.and Sato: "Abnormal modulation of cholinergic neurotransmission by endogenous nitric oxide in bronchus of rats with hyperresponsiveness induced by allergen challenge" Jpn.J.Pharmacol.73. 125-132 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba, Y., Arimoto, T., Yoshikawa, T.and Misawa, M.: "Elevated nitric oxide synthase activity at antigen-induced airway hyperresponsiveness in rats" Am.J.Respir.Cell Mol.Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Chiba,Y.& Misawa,M.: "Characferistic of muscarinic cholinoceptors in airways of antigeninduced airway hyperrespongiveness" Comp.Biochem.Physiol.111C. 351-357 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chiba,Y.&Misawa,M.: "Alferation in Ca^<2+> availability invdved in rat antigenically-induced airway hyperresponsiveness" Eur.J.Pharmacol.278. 79-82 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chiba,Y.& Misawa,M.: "Inhibition of neutral endopeptidase increases airway responsiveness to ACh in nonsensitized normal rats" J.Appl.Physiol.78. 394-402 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chiba,Y.&Misawa,M.: "Charaoferistics of β-adrenoceptors in airways of antigen-induced airway hyperresponsive rats" Pathophysiology. 3. 157-161 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chiba, Y.: "Antigen-induced airwag hyperresbonsiveness is associated with airway tissue NEP hypoactivity in rats" Life Sci.55. 1919-1928 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chiba, Y.: "Inhibition of neutral endopeptidase increases airway responsiveness to ACh in nonsensitized normal rats" J. Appl. Physiol. 78. 394-402 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chiba, Y.: "Characteristic of muscarinic cholinoceptors in airways of antigen-induced airway hyperresponsive rats" Comp. Biochem. Physiol.111. 351-357 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chiba, Y.: "Alteration in Ca^<2+> availavility involved in rat antigenically-induced airway hyperresponsiveness" Eur. J. Pharmacol.278. 79-82 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Misawa, M.: "Abnormal modulation of cholinergic neurotransmission by opioid in hyperresponsive brolchus of rats" Gen. Pharmacol.(accepted).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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