Investigation of physiological functions of a 3-mercaptopyruvate sulfurtransferase
| Project/Area Number |
07672394
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | MEIJI COLLEGE OF PHARMACY |
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Principal Investigator |
ISHII Kazuyuki FACULTY OF PHARMACY MEIJI COLLEGE OF PHARMACY ASSISTANT PROFESSOR, 薬学部, 講師 (90158741)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | human genome cloning / cDNA cloning / 3-mercaptopyruvate sulfurtransferase / rhodanese / expression |
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| Research Abstract |
1. A complete amino acid structure of human liver 3-mercaptopyruvate sulfurtransferase (3-MST,EC2.8.1.2) was determined by sequence analysis of cDNA and purified enzyme. The enzyme consists of 297-amino acid residues with a calculated molecular mass of 33,176.7daltons. Sequence identity in cDNA and the deduced amino acid sequence are 66.9 and 59,7% respectivity, between human 3-MST and rhodanese. By their entire sequence similarity 3-MST and rhodanese are confirmed to be evolutionarily related enzyme. When the 3-MST cDNA was transiently expressed in Escherchia coli and Cos7 cells, the 3-MST activity increased 20-fold and 45-fold, respectively.
2. cDNA for the human rhodanese (thiosulfate ; cyanide sulfurtransuferase, EC 2.8.1.1) was cloned from a human fetal liver cDNA library. Sequencing of the cDNA revealed an open reading frame that encodes a 297-residue polypeptide with a calculated mass of 33427 daltons. When the rhodanese cDNA was transiently expressed in Escherchia coli and Cos7 cells, the rhodanese activity increased 40-fold and 150-fold, respectively. Sequence homology analysis showed that the human rhodanese is 89.6% identical to bovine, 90.2% identical to rat, 91.2% identical to mouse and Chinese hamster, and 71.4% similar to avian counterparts, respectively, and that rhodanese was highly conserved across evolution.
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Report
(3 results)
Research Products
(6 results)
