Analysis of membrane・bound form in alkaline phosphatase induced by short chain fatty acid.
| Project/Area Number |
07672403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | Mukogawa Women's University |
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Principal Investigator |
NAKABAYASHI Toshikatsu Mukogawa Women's University, Department of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (30128665)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | short chain fatty acied / alkaline phosphatase / membrane-bound form |
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| Research Abstract |
The results of this project are as follows :
1. When cultured cells or tissues which express kidney, bone or small intestinal types of alkaline phosphatase (ALP) were treated with phosphatidylinositol・specific phospholipase C (PI-PLC) or glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD), ALP was completely solubilized.
2. Among the HeLa cells tested, HeLa 229 cells showed the highest ability of ALP induction by sodium butyrate (SB). This ALP induction is considered from a change in the enzymatic property. Using a specific antibody and cell sorter, the present site of newly synthesized ALP was whown to be the cell surface. Furthermore, ALP activity was suppressed in the presence of reagents which inhibit the transcription or protein synthesis. These data indicate that ALP induction in HeLa 229 cells by SB is due to changes in the transcriptional level and amount in the synthesis.
3. When HeLa 229 cells were treated with PI-PLC or GP-PLD,the ratio of solubilization of ALP with or without SB treatment was about 10-30 or 50%, respectively. These findings indicate that ALP forms having different sensitivities against PI-PLC or GPI-PLD are present in HeLa 229 cells.
These findings indicate that the membrane-bound form of ALP in HeLa 229 cells expressed by placental type shows diversity compared with those of cultured cells or tissues expressed by kidney, bone or small intestinal types of ALP.
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Report
(3 results)
Research Products
(6 results)
