• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Human genetic analysis on the regulation of Duffy gene expression.

Research Project

Project/Area Number 07672451
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionJichi Medical School

Principal Investigator

IWAMOTO Sadahiko  Jichi Medical School, Dept.of Medicine, Assis.Proff., 医学部, 助教授 (10232711)

Co-Investigator(Kenkyū-buntansha) KAJII Eiji  Jichi Medical School, Dept.of Medicine, Proffessor, 医学部, 教授 (40204391)
OKUDA Hiroshi  Jichi Medical School, Dept.of Medicine, Assis.Lect., 医学部, 助手 (50285772)
土田 修一  自治医科大学, 医学部, 講師 (20217326)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
KeywordsDuffy blood group / Regulation of gene expression / Molecular evolution of gene
Research Abstract

The genomic structure of Duffy gene has been shown not to be split by introns, even in its 5' and 3' untranslated regions. The genes of IL-8 receptors, which are the highly homologous genes of Duffy gene, are not split by introns within the coding regions but are split in the 5' untranslated regions. Then we reanalyzed the transcriptional start position by 5'-rapid amplification of cDNA ends (5'-RACE), and identified a novel first exon and spliced form mRNA that was predominant transcript in both erythroid and postcapillary venule endothelium. The 5' flanking region of the novel first exon was regarded as the transcription controlling unit for both tissues. However, the transcriptional start position in endotherium was 48 bases upstream from that of the erythroid cells. And the 48 bases included an inverted consensus binding site for the GATA transcription factor. We found homozygous one base substitution at the GATA motif in black individuals with Fy (a-b-) phenotype. The Fy (a-b-) in … More dividuals have been shown not to produce Duffy mRNA in the bone marrow, but to produce it in endothelial cells. The tissue-specific lacking of expression in Fy (a-b-) indicates that the transcription control of Duffy gene is under tight tissue-specific regulation. The 5' flanking region of the novel first exon was inserted in the upstream of CAT reporter gene and was analyzed the transcription controlling efficiency. The promotor sequence of Duffy positives efficiently transcribed the reporter gene in both erythroid and endothelial cells. When one base substitution of Duffy negatives was introduced in the GATA motif, the promotor activity in erythroid cells was completely diminished. These data clearly explain the erythroid specific disruption of Duffy gene expression in black type Fy (a-b-). The selective pressure other than Plasmodium vivax have to be studied in the future on the saving of Duffy glycoprotein along endothelial cells in Fy (a-b-) individuals and the fixing the phenotype in West Africa. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Sadahiko,Iwamoto: "Genomic organization of the glycoprotein D gene:Duffy blood group Fy^a/Fy^b alloantigen system is associated with a polymorphismat the 44 amino acid residue." Boold. 85. 622-626 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 池本卯典: "赤血球型をコードする遺伝子." 遺伝. 49. 63-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sadahiko,Iwamoto: "Identification of a novel exon and spliced form of Duffy mRNA that is the predominant transcript in both erythroid and postcapillary venule endothelium." Blood. 87. 378-385 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sadahiko,Iwamoto: "Characterization of the Duffy gene promotor:Evidence for tissue specific abolishment of expression in Fy(a-b-)of black individuals." Biochem Biophys Res Commun. 222. 852-859 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sigenori,Ikemoto: "Molecular genetic basis of red cell markers and its forensic application." Forensic Sci Int. 80. 147-161 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 李剣平: "GTリピートのSSCP解析を用いたDuffy式血液型遺伝子の分子進化の検討." 生物物理化学. 40. 309-312 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jianping,Li: "Dinucleotide repeat in the 3′ flanking region provides a clue to the molecular evolution of the Duffy gene.(in press)" Hum Genet.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toshinori,Omi: "Identification of the truncated Duffy mRNAs in erythroid cells.(in press)" Int J Hematol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Iwamoto, T.Omi, E.Kajii, S.Ikemoto: "Genomic organization of the glycoprotein D gene : Duffy blood group Fya/Fyb alloantigen system is associated with a polymorphism at the 44-amino acid residue." Blood. 85. 622-626 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Iwamoto, J.Li, T.Omi, S.Ikemoto, E.Kajii: "Identification of a novel exon and spliced form of Duffy mRNA that is the predominant transcript in both erythroid and postcapillary venule endothelium." Blood. 87. 378-385 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Iwamoto, J.Li, N.Sugimoto, H.Okuda, E.Kajii: "Characterization of the Duffy gene promotor : Evidence for tissue specific abolishment of expression in Fy (a-b-) of black individuals." Biochem Biophys Res Commun. 222. 852-859 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikemoto S.Iwamoto S,tsuchida S,Goto K,Oyamada T,Kajii E: "Molecular genetic basis of red cell markers and its forensic application." Forensic Sci Int. 80. 147-161 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Li, S.Iwamoto, E.Kajii: "Molecular evolution of Duffy gene." Jpn J Electroph. 40. 309-312 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Li, S.Iwamoto, N.Sugimoto, H.Okuda, E.Kajii: "Dinucleotide repeat in the 3' flanking region provides a clue to the molecular evolution of the Duffy gene." Hum Genet. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Omi, S.Iwamoto, E.Kajii: "Identification of the truncated Duffy mRNAs in erythroid cells." Int J Hematol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sadahiko,Iwamoto: "Genomic organization of the glycoprotein D gene : Duffy blood group Fy^a/Fy^b alloantigen system is associated with a polymorphism at the 44-amino acid residue." Blood. 85. 622-626 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sadahiko,Iwamoto: "Identification of a novel exon and spliced form of Duffy mRNA that is the predominant transcript in both erythoid and postcapillary venule endothelium." Blood. 87. 378-385 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sadahiko,Iwamoto: "Characterization of the Duffy gene promotor : Evidence for tissue specific abolishment of expression in Fy(a-b-) of black individuals." Biochem Biophys Res Commun. 222. 852-859 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Jianping ,Li: "Dinucleotide repeat in the 3′ flanking region provides a clue to the molecular evolution of the Duffy gene." Hum Genet. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 李剣平: "GTリピートのSSCP解析を用いたDuffy式血液型遺伝子の分子進化の検討." 生物物理化学. 40. 309-312 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sigenori,Ikemoto: "Molecular genetic basis of red cell markers and its forensic application." Forensic Sci Int. 80. 147-161 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sadahiko, IWAMOTO: "Genomic organization of the glycoprotein D gene : Duffy blood group Fy^a/Fy^b alloantigen system is associated wityh a polymorphism at the 44 amino acid residue." Blood. 85. 622-626 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sadahiko, IWAMOTO: "Identilication of a novel exon and spliced form of Duffy mRNA that is the predominnt transcript in both erythroid and postcapillary venule endothelium." Blood. 87. 378-385 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi