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Genetical and epidemiological analysis of enterococci resistant to vancomycin

Research Project

Project/Area Number 07672492
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionUniversity of the Ryukyus (1996)
Kumamoto University (1995)

Principal Investigator

YAMANE Nobuhisa  University of the Ryukyus, Faculty of Medicine, Department of Laboratory Medicine, Professor, 医学部, 教授 (80125682)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsVancomycin-resistant enterococci / VanA resistance / VanB resistance / antimicrobial susceptibility testing / polymerase chain reaction / 耐性スクリーニング培地 / プラスミド解析 / パルス・フィールド電気泳動 / バンコマイシン / 腸球菌 / 耐性伝達 / 遺伝子解析 / 薬剤感受性検査 / 疫学調査
Research Abstract

The study was conducted to evaluate the test methods available in Japan to discriminate between the isolates of enterococci resistant to vancomycin (VRE) and those susceptible (VSE). Among the phenotypic test methods evaluated, an agar screening method which utilized 8 mug per ml of vancomycin in Mueller-Hinton agar appeared to have a sufficient accuracy, the results indicating 98.3% of sensitivity and 100% of specificity to detect VRE.Also, all the VRE isolates were interpreted as being resistant or intermediate by the E test when the results were read after 48 hr-incubation. Whereas, two disk diffusion tests, Showa disk and NCCLS-based Sensi-disc, were evaluated, but both failed to discriminate between VRE and VSE,in particular, between the isolates with vanB positive and negative. The automated system, Vitek GPS-TA produced high frequencies of very majior errors ; 8.7% for vanA positives and 58% for vanB positives.
A total of 1,214 enterococcal isolates from multisite laboratories in Japan, comprising 7 different species, were first tested onto agar screening test plates, but none of isolates represented phenotypic vancomycin resistance.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 山根 誠久: "バンコマイシン耐性腸球菌検出のための各種薬剤感受性試験の検討" 臨床病理. 45(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nobuhisa YAMANE: "Laboratory-evaluation of antimicrobial susceptibility testings to detect vancomycin-resistant enterococci" Clin. Pathol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山根 誠久: "バンコマイシン耐性腸球菌検出のための各種薬剤感受性試験の検討" 臨床病理. 45(印刷中). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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