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Detection of HIV-RNA by Quantitative PCR and Use as a Prognostic Marker and for Evaluating Antiretroviral Therapy

Research Project

Project/Area Number 07672494
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionUniversity of Shizuoka

Principal Investigator

MORITA Tamotsu  University of Shizuoka, Institute for Environmental Sciences, Professor, 環境科学研究所, 教授 (40046264)

Co-Investigator(Kenkyū-buntansha) NAITOU Hirotaka  University of Shizuoka, Institute for Environmental Sciences, Research Associate, 環境科学研究所, 助手 (30254262)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordshemophiliacs / serum HIV-1 RNA content / competitive PCR / HIV infection prognosis / treatment evaluation / treatment change / HIV surrogate marker / long term non-progressor / プロテアーゼ阻害剤 / 3剤併用療法 / competitive-PCR法 / d4T / 治療開始時期
Research Abstract

Quantitative detection of HIV-1 RNA in sera was performed by competitive polymerase chain reaction (PCR). The amount of HIV-1 genomic RNA in sera is the most direct marker for patient prognosis and monitoring the efficacy of antiretroviral therapy.
HIV-1 RNA in frozen-stored sera from 24 seropositive patients (21 hemophiliacs and 3 homosexual men) under the treatment at Shizuoka Children's Hospital were periodically quantified by cometitive PCR.The copy numbers of HIV-1 RNA were compared with patient's clinical status and clinical surrogate markers such as CD4 lymphocyte count, CD4/CD8 lymphocytes ratio, IgA and IgG levels, beta2-microglobulin level and p24 antigen level. Close correlation was observed between the amount of viral RNA in sera and patients' clinical status. Copy numbers of viral RNA began to increase in log order before deterioration of patient's symptomes but decreased significantly within a few days after the antiretroviral treatments. Other clinical surrogate markers including CD4 lymphocyte count did not show remarkable changes. Detection of p24 antigen was unreliable throughout their clinical courses. The monitoring of HIV-1 RNA contents in sera is very useful for the prognosis of HIV-infection and the evaluation of the treatment.
In the case of two AC patients seemed to be long term non-progressor, viral RNA content was kept less than 100 copies/ml serum. Seven in 12 patients receiving combination therapy with protease inhibitors and reverse transcriptase inhibitors also showed very low viral RNA contents which were less than detection limit of the Amplicor HIV Monitor (more than 200 copies, Roche Diagnostic Systems).

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Tamotsu Morita et al.: "The amount of HIV-1 RNA in sera from HIV-infected hemophiliacs and their clinical courses" AIDS Res.Newslett.-Proceeding of the 8th Congress of the Japanese Society for AIDS Research-. 124- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuo Horikoshi et al.: "The comparison of the content of HIV-1 RNA in sera with serologic markers and clinical course" AIDS Res.Newslett.-Proceeding of the 9th Congress of the Japanese Society for AIDS Research-. 16- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akemi Yamazaki et al.: "Quantitative examination of HIV-1 RNA using branched DNA assay" AIDS Res.Newslett.-Proceeding of the 9th Congress of the Japanese Society for AIDS Research-. 94- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 内藤 博敬 等: "Conpetitive-PCR法によるHIV-RNAのHIVマーカーおよび臨床経過" 日本細菌学雑誌. 51. 320- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirotaka Naitou et al.: "Quantitative detection of HIV-1 RNA by PCR and use as a prognostic marker and for evaluating antiretroviral therapy" Biol.Pharm.Bull.20. 1317-1320 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshifumi Takashima et al.: "The clinical efficacy of stavudine (d4T) alone in 8 HIV-infected individuals" AIDS Res.Newslett.-Proceeding of the 10th Congress of the Japanese Society for AIDS Research-. 147- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tamotsu Morita et al.: "The amount of HIV-1 RNA in sera from HIV-infected hemophiliacs and their clinical courses" AIDS Res.Newslett. -Proceeding of the 8th Congress of the Japanese Society for AIDS Research-. 124 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuo Horikoshi et al.: "The comparison of the content of HIV-1 RNA in sera with serologic markers and clinical course" AIDS Res.Newslett. -Proceeding of the 9th Congress of the Japanese Society for AIDS Research-. 16 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akemi Yamazaki et al.: "Quantitative examination of HIV-1 RNA using branched DNA assay" AIDS Res.Newslett. -Proceeding of the 9th Congress of the Japanese Society for AIDS Research-. 94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirotaka Naitou et al.: "Quantitative detection of HIV-1 RNA by PCR and use as a prognostic marker and for evaluating antiretroviral therapy" Biol.Pharm.Bull.20. 1317-1320 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshifumi Takashima et al.: "The clinical efficacy of stavudine (d4T) alone in 8 HIV-infected individuals" AIDS Res.Newslett. -Proceeding of the 10th Congress of the Japanese Society for AIDS Research-. 147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hirotaka Naitou et al.: "Quantitative detection of human immunodeficiency virus type 1 RNA by PCR and use as a prognostic marker and for evaluating antiretroviral therapy" Biol.Pharm.Bull.20(12). 1317-1320 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshifumi Takashima et al: "The clinical efficacy of stavudine (d4T) alone in 8 HIV-infected individuals" AIDS Res.Newslett.-Proceeding of the 10th Congress of the Japanese Society for AIDS Research-. 147 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 内藤博敬 等: "血清中 HIV-1 RNA量の経時変化およびHIVマーカーによる逆転写酵素阻害剤の治療効果判定の比較検討" 日本細菌学雑誌. 52(1). 227 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yasuo Horikoshi et al.: "The comparison of the content of HIV-1 RNA in sera with serologic markers and clinical course" AIDS Res. Newslett. -Proceeding of the 9th Congress of the Japanese Society fo AIDS Research-. 16- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akemi Yamazaki et al.: "Quantitative examination of HIV-1 RNA using branched DNA assay" AIDS Res. Newslett. -Proceeding of the 9th Congress of the Japaness Society for AIDS Research-. 94- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 内藤博敬 等: "Conpetitive-PCR法によるHIV-RNAのHIVマーカーおよび臨床経過との比較検討" 日本細菌学雑誌. 51. 320- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Morita,H.Naitou,Y.Horikoshi,K.Amano,J.Mimaya: "The amount of HIV-1 RNA in sera from HIV-infected hemophiliacs and their clinical courses" AIDS Res.Newslett.-Proceeding of the 8th Congress of the Japanese Society for AIDS Research-. 124 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 内藤博敬,森田全: "Conpetive-PCR法によるHIV-RNAの定量的検出と病態進展予測および治療効果判定指標としての応用" 日本細菌学雑誌. 50. 229 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2021-09-01  

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