• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of Antigenic Composition of Calibrator and Serum on Lipoprotein (a) Measurement

Research Project

Project/Area Number 07672497
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionJichi Medical School

Principal Investigator

SAKURABAYASHI Ikunosuke  Jichi Medical School Chairman, Professor, 医学部, 教授 (80049056)

Co-Investigator(Kenkyū-buntansha) KUBO Nobuhiko  Jichi Medical School assistant, 医学部, 助手 (40214994)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordslipoprotein (a) / apo lipoprotein (a) / immunoassay / monoclonal antibody / standardization / lipoprotein(a) / apolipoprotein(a) / standard material / standardization / epitope analysis / gene expression / monoclonal antibody / lipoprotein (a) / apolipoprotein (a) / staudard material / stundardization
Research Abstract

Two direct binding ELISA using anti-Lp (a) /apo (a) antibody was developed to investigate the effects of calibration on Lp (a) value. Paired Lp (a) value of sera from 59 individuals was obtained by the identical ELISA using two calibration curves by each commercial calibrator. The difference between paired Lp (a) value was produced by the reactivity [against the anti-Lp (a) /apo (a) antibody] difference among constructs of the two calibrators. Not only the reactivity against anti-Lp (a) /apo (a) antibody, but also the amount of partial Lp (a) antigen were different among constructs and between the two calibrators suspected that the disagreements between the paired Lp (a) value by the two calibrators could be delived from different transferred Lp (a) value from standards to each constructs of calibrator. We also found the partial Lp (a) antigen was heterogeneous among individuals that may affects on Lp (a) value among different Lp (a) assays. Even it is not complete but it was suspected that not only unifying but also opening the character of standard material and acheive the agreement over the method for transferring Lp (a) value from standard to the calibrator of each assay, are neccessary to reduce the difference in Lp (a) values by different assay methods.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 久保 信彦: "動脈硬化症マーカーとしてのアポ(a)アイソフォーム分析" 生物物理化学. 39(6). 315-320 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 久保 信彦: "技術講座Lp(a)測定法" Medical Technology. 24(12). 1207-1211 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻林 郁之介: "日本臨床病理学会標準委員会・アドホックLp(a)標準小委員会報告I." 臨床病理. 44(3). 287-291 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻林 郁之介: "日本臨床病理学会標準委員会・アドホックLp(a)標準小委員会報告II." 臨床病理. 44(3). 292-296 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻林 郁之介: "国際化学連合(IFCC)Working Group Lp(a)国際標準委員会報告" 臨床病理. 44(3). 297-298 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 櫻林 郁之介: "国際化学連合(IFCC)Working Group Lp(a)国際標準委員会報告II" 臨床病理. 44(3). 299-300 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakurabayashi I,Kubo N,Katayama Y,Abe A,Noma A and Kawai T.: "report of and hoc Lp (a) standardization subcommittee in standardization committee of Japan Society of Clinical Pathology. I.summary of the results of questionnaire to the Lp (a) test reagent makers." Jpn J Clin Pathol. 44 (in Japanese). 287-91 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakurabayashi I,et al.: "Report of and hoc Lp (a) standardization subcommittee in standardization committee of Japan Society of Clinical Pathology. II.results of the survey for the correction of inter-laboratory variations by a unified reference material." Jpn J Clin Pathol. 44 (in Japanese). 292-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakurabayashi, I.: "Ist reprot of the International Committee meeting of IFCC working group on Lp (a) standardization." Jpn J Clin Pathol. 44 (in Japanese). 297-298 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakurabayashi, I.: "3rd report of the International Committee meeting of IFCC working group on Lp (a) standardization." Jpan J Clin Pathol. 45 (in Japanese). 286-287 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.YAMADA et al: "Design of the antibody production by synthetic peptide and establishment of Lp(a) measurement" Preceedings of the XVII world congress of anatomic and clinical pathology. 539-543 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保信彦: "Lp(a)は動脈硬化と直接関連しているか-動脈硬化単での役割と血中濃度測定の有用性" 臨床病理. 42. 1119-1127 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保信彦,他: "動脈硬化症マーカーとしてのアポ(a)アイソフォーム分析" 生物物理化学. 39. 315-320 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保信彦,他: "Lp(a)の働きと動脈硬化-動脈硬化発症の独立した危険因子" 日本臨床化学関東支部会誌. 2. 48-49 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] 久保信彦,他: "Lp(a)の比濁法による測定の基礎的検討" 臨床検査機器・試薬. 17. 369-372 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. YAMADA et. al: "Design of the antibody production by synthetic peptides and estiblisblislunent on Lp(a) measurement" Preceedings of the XVII world ccngress of anatonic and clinical phthology. 539-543 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久保信彦 他: "Lp(a)は動脈硬化と直接関連しているか-動脈硬化等での役割と血中濃度測定の有用性" 臨床病理. 42. 1119-1127 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久保信彦: "動脈硬化症マーカーとしてのアポ(a)アイソフォーム分析" 生物物理化学. 39. 315-320 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久保信彦 他: "Lp(a)の働きと動脈硬化-動脈硬化発症の独立した危険因子" 日本臨床化学会関東支部会誌. 2. 48-49 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久保信彦: "Lp(a)の比濁法による測定の基礎的検討" 臨床検査危機・試薬. 17. 369-371 (1994)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi