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Correlation Between Freeze-tolerance of Bakers' Yeasts and Their Cellular Content of Trehalose

Research Project

Project/Area Number 07680019
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 家政学
Research InstitutionNara Women's University

Principal Investigator

KAWAI Hiroyasu  Nara Women's University, Department of Food Science and Nutrition, Professor, 生活環境学部, 教授 (80026525)

Co-Investigator(Kenkyū-buntansha) ISOBE Yuka  Nara Women's University, Department of Food Science and Nutrition, Assistant, 生活環境学部, 助手 (80218544)
YOKOIGAWA Kumio  Nara Women's University, Department of Food Science and Nutrition, Associate Pro, 生活環境学部, 助教授 (60230637)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsBakers' Yeast / Freeze-tolerant Yeast / Mechanism of Freeze-tolerance / Torulaspora delbrueckii / Trehalose / Saccharomyces cerevisiae / Torulaspora delfrueckii / Torulaspora, delbrueckii
Research Abstract

This study was undertaken to clarify the correlation between freeze-tolerance of bakers' yeasts and their intracellular trehalose, a reserve carbohydrate in yeast. 1. The amount of trehalose in a freeze-tolerant cells of Torulaspora delbrueckii was higher than that of a freeze-sensitive mutant. The result suggested that the freeze-tolerance of yeast has a close relationship to its trehalose content. 2. The trehalose content of growing yeast cells was increased by a heat-shock, i.e., a temporal increase in culture temperature, and these yeast cells were found to have higher survival ratio after freeze-thawing. This indicated that intracellular trehalose of yeast has an important role to lower the freeze-injury of yeast. 3. There were found no clear correlation between trehalose content of commercial compressed bakers' yeasts belonging to Saccharomyces cerevisiae and their freeze-tolerance, but their cultured yeast cellsshowed a positive relationship between them. 4. A trehalase-deficient mutant of T.delbrueckii had a high trehalose content, while a mutant having a weak trehalose-phosphate synthetase activity had a low trehalose content. These results reflected a close relationship between freeze-tolerance and cellular content of trehalose. 5. It was found that freeze-tolerant strain had a higher trehalose-phoshpate synthetase activity and a lower neutral trehalase activity than that of freeze-sensitive strain, the results indicating that those enzyme activities were closely related to the amount of cellular trehalose and freeze-tolerance of yeast.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] YOKO MURAKAMI: "Lipid Composition of a freeze-tolerant yeast, Torulaspora dilbweckii and its freeze-sensitine mutant" Applied Microbiology & Bistechnology. 44. 167-171 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] KUMIO YOKOIGAWA: "Trehalase Activity and Treholose Content on a Freege-tolerant yeast, Torulaspora delbiueckii and Its Freeze-sensitwe Matant" Biosecence, Biotechnology & Biochemistry. 59. 2143-2145 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YOKO MURAKAMI: "Lipid Composition of Commerciae Bakers' yeasts Having Difkerent Freeze-toleiance in Frozen Dough" Bioscience, Biotechnology of Biochenmistry. 60. 1874-1876 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoko Murakami: "Lipid Composition of a Freeze-tolerant Yeast, Torulaspora delbrueckii and Its Freeze-sensitive Mutant" Appl.Microbiol.Biotechnol.44. 167-168 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kumio Yokoigawa: "Trehalase Activity and Trehalose Content in a Freeze-tolerant Yeast, Torulaspora delbrueckii and Its Freeze-sensitive Mutant" Bisci.Biotech.Biochem.59. 2143-2145 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoko Murakami: "Lipid Composition of Commercial Bakers' Yeasts Having Different Freeze-tolerance in Frozen Dough." Biosci.Biotech.Biochem.60. 1874-1876 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YOKO MURAKAMI: "Lipid Composition of a freeze-tolerant yeast,Torulaspora delfrueckii and its freeze-sensitive mutant" Applied Microbiology of Biotechnology. 44. 167-171 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] KUMIO YOKOIGAWA: "Trehalase Activity and Trehalose Content in a Freeze-tolerant yeast,Torulospora delfrueckii and Its Freeze-sensitive Mutant" Bioscience,Biotechnology & Biochemistry. 59. 2143-2145 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] YOKO MURAKAMI: "Lipid Conposition of a freege-tolerant, yeast, Torulaspora delbrueckii, and its freege-sensitive mutant" Applied Nicrobiology = Biotechnology. 44. 167-171 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] KUMIO YOKOIGAWA: "Trekalase Aetivity and Trekalose Content in a Freege-tolerant yeast, Torulaspora delbrueckii, and Its Freege-sensitive Mutant" Bioseience,Biotechnology and Biochemistry. 59. 2143-2145 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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