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Validation study of assays alternative to animal experiments from a statistical viewpoint

Research Project

Project/Area Number 07680327
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Statistical science
Research InstitutionScience University of Tokyo

Principal Investigator

YOSHIMURA Isao  Faculty of Engineering, Science University of Tokyo, Professor, 工学部, 教授 (30010797)

Co-Investigator(Kenkyū-buntansha) 岩下 登志也  東京理科大学, 工学部, 助手 (20266919)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsAlternative assay / Draize score / Eye irritation / Dose response / Validation / ED50 / ED_<50>推定 / Draize評点 / 細胞毒性試験 / 予測 / データクリーニング
Research Abstract

There are three statistical issues to be addressed in the validation study of assays alternative to animal experiments. They are (1) the reliability and feasibility of assays, (2) the features of in vivo tests to be alternated, and (3) the degree of correlation or the predictability of in vitro assays to in vivo tests. Regarding (1), this reserch contributed to the validation study sonducted by the Japanese Society of Alternatives to Animal Experiments by statistically analyzing the data collected by the validation study and induced the conclusion that the Crystal Violet Assay is the most reliable one through the data analysis. Regarding (2), this research proposed a method of data analysis, which is referred to as the maximum contrast method, to evaluate toxicity of chemical compounds and identify the dose with no observed toxicity. Regarding (3), this reserch succeeded in identifying a battery of assays optimally predict the Draize eye irritation scores by using the prediction sum of squares as the criterion.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] ISAO YOSHIMURA,AKIRA WAKANA,CHIKOMA HAMADA: "A PERFORMANCE COMPARISON OF MAXIMOM CONTRAST METHODS TO VETCT DOSE DEPENDENCY" Drug Information Jouenal. (to appear). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] ISAO YOSHIMURA,AKIRA WAKANA CHIKUMA HAMADA: "A PERFORMANCE COMPARISON OF MAXIMUM CONTRAST METHODS TO DETECT DOSE DEPENDENCY" Drug Information Journal. (to appear). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] I.Yoshimura他5名: "Evaluation of seven alternative assays on the main ingredients in cosmetias as predictors of Draize eye irritation scores" Toxicology in Vitro. 9. 333-340 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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