Random Amplified Polymorphic DNA (RAPD) Analyzes for Discriminating Genotypes of Microcystis Cyanobactcria and Its Application for the Management of Water Quality of Lakes
| Project/Area Number |
07680551
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental dynamic analysis
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| Research Institution | Ibaraki University |
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Principal Investigator |
TAKAMURA Yoshichika School of Agriculture, Ibaraki University, Professor, 農学部, 教授 (20007678)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIHARA Hirofumi School of Agriculture, Ibaraki University, Associate Professor, 農学部, 助教授 (10260465)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | RAPD analysis / DNA polymorphism / Microcystis / cyanobacterium / allozyme genotype / RAPD / 水の華(アオコ) / シアノバクテリア / 識別同定 / DNA多型解析 / アロザイム・ゲノタイプ / 識別同定法 / 富栄養湖 |
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| Research Abstract |
Random amplified polymorphic DNA (RAPD) analysis was applied to the discrimination of gcnotypes in five species of Microcystis cyanobacteria. Strains of each group possessing the identical allozyme genotype gave similar RAPD patterns characterized by the respective group. On the other hand, any similarities in RAPD patterns were not observed among strains of which allozyme genotypes were different. A good accordance between the RAPD analysis and allozyme divergence indicated a high reliability of the both methods for discrimination of the affiliated group of Microcystis. Several amplified DNA fragments, which were expected as markers for a particular taxon with identical allozyme genotype, were also observed on the RAPD patterns. Genetic homogenieties of M.novacekii, M.viridis and M.wesenbergii were shown by RAPD analysis as well as allozyme genotypes. However, significant variations were observed in M.aeruginosa and M.ichthyoblabe in the levels of DNA and proteins (allozymes).
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Report
(4 results)
Research Products
(4 results)
