| Project/Area Number |
07680592
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境影響評価(含放射線生物学)
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| Research Institution | OKAYAMA UNIVERSITY OF SCIENCE |
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Principal Investigator |
NOGAMI Yusaku OKAYAMA UNIV.OF SCIENCE,SCIENCES,PROFESSOR, 理学部, 教授 (00172768)
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| Co-Investigator(Kenkyū-buntansha) |
KIRA Shohei OKAYAMA UNIV.MEDICAL SCHOOL,MEDICAL,LECTURER, 医学部, 講師 (50033212)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | WATER ENVIRONMENT / TWA (Time Weighted Average) / MUTAGEN / BENZO (a) PYRENE / CAPILLARY ACTION |
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| Research Abstract |
Benzo (a) pyrene (BaP) , a well-known carcinogen/mutagen, is detected in aquatic biota and sediment at rather higher level than that found in water. To investigate the dynamics of this chemical in environment, a time-weighted average concentration (TWA) is more practical determinant than the level that is detected in a spot-sampled water. Because the chronic effects on the environmental matrix including biota mostly depend on the prolonged presence of pollutant. In this study, we testeda newly developed sampling device that collect pollutants continuously for more than 1 week and the levelsare expressed as TWA.We set up an experimental water system with dosing column which supplies BaP into water in an aquarium continuously and maintained the level at 0.2ppb. But it was difficult to obtain a desired level of BaP by the system. The sampler used here consisted of two major parts. A glass pipe filled with the blue rayon (BR) as an adsorbent (sampling pipe part) was connected to an airtigh
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t chamber that contained silica-gel as a desiccant (sucker chamber part). The sampler was fixed on a float and placed on the surface of the aquarium immersing the end of sampling pipe in water, then left for a designated period of time. The water vapor pressure is reduced by silica-gel, so the test-water is slowly sucked up from the bottom end of the pipe due to the capillary potential. The water volume passed through the pipe was estimated by weight difference of silica-gel. Transfer of BaP also occurs and is adsorbed on BR in the pipe. Repeated test showed 25 ml of water passed through the pipe for 1 week and the sampling rate was stable. BaP adsorbed on BR was recovered by the extraction with methanol/ammonia solution and analyzed using HPLC with fluorescent detection. BaP in the aquarium was also quantified from spot-samples by extraction with petroleum ether as time lapsed. A TWA calculated from a recovered amount of BaP by dividing by the volume of water passed through the pipe and an average concentration of BaP in the aquarium coincided with each other as long as 1-week sampling. In case of 10-day sampling or longer, the obtained TWA of BaP showed lower level than that we expected. BaP that adsorbed on BR might decompose during sampling, yet reasons should be investigated. The performance of this sampling device at a field water is still underway, since the level of BaP might be far lower than 0.2 ppb under a normal condition of water environment. Less
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