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Design, structures and functions of stabilized artificial ion channels

Research Project

Project/Area Number 07680629
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

IIDA Akira  Kyoto Univ., Fac.of Pharm.Sci., Associate Professor, 薬学部, 助教授 (40202816)

Co-Investigator(Kenkyū-buntansha) NAGAOKA Yasuo  Kyoto Univ., Fac.of Pharm.Sci., Instructor, 薬学部, 助手 (90243039)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsartificial ion channel / peptide bunndle / Trichoderma polysporum / trichosporin / peptaibol / voltage-dependent channel / imperfect fungus / barrel stave
Research Abstract

20-residue peptaibols, trichosporins-B produced by the fungus Trichoderma polysporum, forms voltage-dependent ion channels in planar lipid bilayr membranes. To clarify primary factors for channel stabilization and to develop new functional materials such as artificial ion channels, trichosporin derivatives including a template-assembled dimer and tetramer and two disulfide-linked dimers were synthesized and their channel-forming properties were investigated.
The results obtained in this study were summarized as followes ;
1.The chennels induced by truncated and elongated derivertives were unstable, suggesting that peptide length fitting the membrane thickness is quite important.
2.The Pro-kinked structure is not essential for voltage-dependent channel formation and very important for forming stable channels with large pore size.
3.Peptide lipophilicity promotes peptide aggregation and this is not in accordance with channel stability.
4.Stability of channels is influenced by the states of supermolecules consisting of pepides, lipids and water.
5.All chemically assembled peptides (helix bundle) form very long-lasting channels, supporting that peptaibol channels can be explained through the barrel stave model.
6.Success in formation of the stable channels may lead to developing novel functional materials.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Yasuo Nagaoka: "Ion-channel-forming and Catecholamine-releasing of Elongated and Truncated Analogues of Trichosporin-B" J. Chem Soc. , Chem. Commu.1995. 2203-2204 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Role of proline residue in the channel-forming and catecholamine-releasing activities of the peptaibol, Trichosporin-B-Vla" Biochim. Biophys. Acta. 1283. 31-36 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Role of Gln^7 in the ion-channel-forming properties of the trichosporin-B-Vla" J. Chem Soc. , Chem. Commu.1996. 1079-1080 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shun-ichi Wada: "ION CHANNNEL-FORMING PROPERTY OF TRICHOROVIN-XII, AN II-RESIDUE PEPTAIBOL FROM THE FUNGUS TRICHODERMA VIRIDE, IN PLANAR LIPID BILAYER MEMBRANES" Bioorg. Med. Chem. Lett.6. 2275-2278 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Ion Channel Properties of Disulfide-Linked Dimer Derivative of Paptaibol, Trichosporin-B-Vla" Peptide Chemistry 1996. 189-192 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Ion-channel-forming and Catecholamine-releasing Activities of Elongated and Truncated Analogues of Trichosporin-B" J.Chem Soc., Chem.Commu.1995. 2203-2204 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Role of proline residue in the channel-forming and catecholamine-releasing activities of the peptaibol, Trichosporin-B-VIa" Biochim.Biophys.Acta. 1283. 31-36 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Role of Gln^7 in the ion-channel-forming properties of the trichosporin-B-VIa" J.Chem Soc., Chem.Commu.1996. 1079-1080 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shun-ichi Wada: "ION CHANNNEL-FORMING PROPERTY OF TRICHOROVIN-XII,AN 11-RESIDUE PEPTAIBOL FROM THE FUNGUS TRICHODERMA VIRIDE,IN PLANAR LIPID BILAYER MEMBRANES" Bioong.Med Chem.Lett.6. 2275-2278 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Ion Channel Properties of Disulfide-Linked Dimer Derivative of Peptaibol, Trichosporin-B-VIa" Peptide Chemistry 1996. 189-192 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Nagaoka: "Ion-channel-forming and Catecholamine-releasing Activities of Elongated and Truncated Analogues of Trichosporin-B" J.Chem Soc.,Chem.Commu.1995. 2203-2204 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuo Nagaoka: "Role of proline residue in the channel-forming and catecholamine-releasing activities of the peptaibol,Trichosporin-B-VIa" Biochim.Biophys.Acta. 1283. 31-36 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuo Nagaoka: "Role of Cln^7 in the ion-channel-forming properties of the trichosporin-B-VIa" J.Chem Soc.,Chem.Commu.1996. 1079-1080 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shun-ichi Wada: "ION CHANNNEL-FORMING PROPERTY OF TRICHOROVIN-XII,AN 11-RESIDUE PEPTAIBOL FROM THE FUNGUS TRICHODERMA VIRIDE,IN PLANAR LIPID BILAYER MEMBRANES" Bioorg.Med.Chem.Lett.6. 2275-2278 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuo Nagaoka: "Ion Channel Properties of Disulfide-Linked Dimer Derivative of Peptaibol,Trichosporin-B-VIa" Peptide Chemistry. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuo Nagaoka: "Ion-Channel-Forming and Carecholamine-releasing Activities of Elongated and Truncuted Aualogues of Trichosporin-B" J. Chem. Soc., Chem. Commun.1995. 2203-2204 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yasuo Nagaoka: "Role of Glm^7 in the Ion-Channel-Forming Properties of a peptaibol, Trichopirim B-Vla" J. Chem. Soc., Chem. Commun.1996(発表予定). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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