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Molecular Design and Structure-Function of Ion Transfer Peptides : Investigation of structural stabilizing factor, Trp and Functional Factor, Glu

Research Project

Project/Area Number 07680634
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionSAGA UNIVERSITY

Principal Investigator

KONDO Michio  Saga Univ.Depart.of Chemistry Professor, 理工学部, 教授 (30039250)

Co-Investigator(Kenkyū-buntansha) KODAMA Hiroaki  Saga Univ.Depart.of Chemistry Associate Professor, 理工学部, 助教授 (80205418)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsGramicidin analog / Aib / CD spectra / Conformation / Ion-channel / Hydrophobic interaction / Solid-phase synthesis / グラミシジンAibアナログ / 疎水性自己会合
Research Abstract

Peptaibols form alpha-helical structures leading to voltage-dependent ion channels in lipid membranes. It has been confirmed that 2-aminoisobutyric acid (Aib)-containing peptides tend to take alpha-helix conformations and form voltage-dependent channels in lipid bilayrs. In the present study, amphiphilic helical Aib-containing peptides with various chain lengths, Ac- (Aib-Lys-Aib-Ala) n-NH2 (n=1-5 ; named as BKBA-4,8,12,16 and 20, respectively) have been designed to investigate the aggregation and transmembrane orientation of Aib-containing helical motifs in lipid bilayrs.
Peptide synthesis was carried out by a solid-phase method. CD spectra of BKBA-4 and BKBA-8 in the buffer exhibited a weak band at 200 nm, representative of non-ordered structure. CD spectra of peptides having longer peptide chains, BKBA-12,16 and 20, exhibit the typical double minimum bands, characteristic of alpha-helix. The helicities of the peptides increased with increasing of chain length. In particular, BKBA-16 and 20 took almost complete a-helical structures (77 and 85%, respectively). In TFE,BKBA-12,16 and 20 also displayd significant a-helical contents. From comparing the CD spectra of the peptides in the buffer and TFE,the band at 208 nm increases dramatically in TFE.The reversal of the ellipticity ratio of 208 to 225 nm in TFE may reflect the transition from an aggregated state in buffer to single stranded helices in TFE.Moreover, the alpha-helical structures were intensified in phospholipid liposomes. Especially, CD characteristics of BKBA-16 and 20 were very close to those of alamethicin. These results suggest that BKBA-16 and 20 may aggregate and form alamethicin-like barrel-stave conformations in phospholipid bilayrs. This structure can be also confirmed by the formation of stable conducting pores in bilays, using patch-clamp methodology.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] M.Kondo et al.: "A Tetrameric Enkephalin Analog for the Putative Multivalent Interaction with Opioid Receptors" Bull.Chem.Soc.Jpn. 68. 3161-3167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kumon et al.: "N-Phosphoarginine Phosphatase (17 kDa) and Alkaline Phosphatase as Protein Arginine Phosphatases" J.Biochem.116. 719-724 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kodama et al.: "Development of a Direct Quantitative Method for N^G-Phosphoarginine Determination in Proteins" Peptides,Chemistry,Structure and Biology.440-441 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kaibara et al.: "Characteristic Interaction of Ca2+ Ions with Elastin Coacervate : Ion Transport Stydy Across Coacervate Layers of alpha-Elastin and Elastin Model Polypeptide,(Val-Pro-Gly-Val-Gly)n" Biopolymers. 39. 189-198 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sakamoto et al.: "Chemical synthesis of phosphrylated peptides of the carboxy-terminal domain of human p53 by a segmant condensation method" Int.J.Pept.Protein Res.48. 429-442 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kondo et al.: "Solid-phase Synthesis and Conformational Analysis of Amphiphilic Aib-containing Helical Peptides" Peptides 1998. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kondo et al.: "A Tetrameric Enkephalin Analog for the Putative Multivalent lnteraction with Opioid Receptors" Bull.Chem.Soc.Jpn. 68. 3161-3167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kumon et al.: "N-Phosphoarginine Phosphatase (17 kDa) and Alkaline Phosphatase as Protein Arginine Phosphatases." J.Biochem.119. 719-724 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kodama et al.: "Development of a Direct Quantitative Method for N^G-Phosphoarginine Determination in Proteins" Peptides, Chemistry, Structure and Biology. 440-441 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kaibara et al.: "Characteristic Interaction of Ca2+Ions with Elastin Coacervate : Ion Transport Stydy Across Coacervate Layrs of alpha-Elastin and Elastin Model Polypeptide, (Val-Pro-Gly-Val-Gly) n" Biopolymers. 39. 189-198 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Sakamoto et al.: "Chemical synthesis of phosphrylated peptides of the carboxy-terminal domain of human p53 by a segmant condensation method" Int.J.Pept.Protein Res.48. 429-442 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kondo et al.: "Solid-phase Synthesis and Conformational Analysis of Amphiphilic Aib-containing Helical Peptides" Peptides 1998. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Kumon et al.: "N-Phosphoarginine Phosphatase (17kDa) and Alkaline Phosphatase as Protein Arginine Phosphatases" J.Biochem.119. 719-724 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yasunaga et al.: "Reversible Affinity Labeling of Opioid Receptors via Disulfide Bonding : Discriminative Labeling of mu and delta Subtypes by Chemically Activated Thiol-containing Enkephalin" J.Biochem.120. 459-465 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Sakaguchi et al.: "Effect of Phosphorylation on Tetramerization of the Tumor Suppressor Protein P53" Peptides,Chemistry,Structure and Biology. 252-254 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Kodama et al.: "Development of a Direct Quantitative Method for NG-Phosphoarginine Determination in Proteins" Peptides,Chemistry,Structure and Biology. 440-441 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Kaibara et al.: "Characteristic Interaction of Ca2+Ions with Elastin Coacervate : Ion Transport Stydy Across Coacervate Layers of alpha-Elastin and Elastin Model Polypeptide,(Val-Pro-Gly-Val-Gly)n" Biopolymers. 39. 189-198 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Sakamoto et al.: "Chemical synthesis of phosphrylated peptides of the carboxy-terminal domain of human p53 by a segmant condensation method" Int.J.Pept.Protein Res.48. 429-442 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kondo et al.: "A Tetrameric Enkephalin Analog for the Putative Multivalent Interaction with Opioid Receptors" Bull.Chem.Soc.Jpn.68. 3161-3167 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N.Shindoh et al.: "New Cysteine Fluorometric Determination for Vegetables and Fruits Based on a Reaction with 2,6-Dichloro-p-benzoquinone and Using Thin-layer Densitometry" Biosci.Biotech.Biochem.59. 108-110 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.J.-Niaraki et al.: "Conformational Studies and Pore-Forming Properties of an α-Aminoisobutyric Acid Analogue of Gramicidin B" J.Chem.Soc.,Perkin Trans 2. 801-808 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Miyazaki et al.: "Role of the Side Chain Orientation of Phe^3 Residue for the Biological Activity of the Chemotactic Peptide fMLP" Peptide Chemistry 1994. 293-296 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Yasunaga et al.: "Specificity and Selectivity in Affinity Labeling of Opioid Receptors by Cysteamine-Containing Enkephalin Analogs" Peptide Chemistry 1994. 333-336 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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