• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular evolution of Galectins and their ligands

Research Project

Project/Area Number 07680659
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionTeikyo University

Principal Investigator

KASAI Ken-ichi  Teikyo Univ., Fac.Pharm.Sci., Professor, 薬学部, 教授 (40001052)

Co-Investigator(Kenkyū-buntansha) ARATA Yoichiro  Teikyo Univ., Fac.Pharm.Sci., Assistant Professor, 薬学部, 助手 (90246017)
HIRABAYASHI Jun  Teikyo Univ., Fac.Pharm.Sci., Lecturer, 薬学部, 講師 (40156691)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsGalectin / Neatode / C.elegans / Glycoconjugate / Lectin / ガクレチン / 皮膚
Research Abstract

The complete structure of the gene for 32-kDa galection of the nematode Caenorhabditis elegans, which is the firstly found invertebrate galectin, was determined. Only two introns were found. The insertion position of one intron corresponds to those found in the cases of vertebrate galectins, while that of the other was unique. We also found another galectin from C.elegans, which corresponds to the proto type of vertebrates, cloned its cDNA,and determined its whole structure. The finding that both vertebrates and C.elegans have a similar set of galectins strongly suggests the existence of common biological roles that shuould be indespensable for multicellular animals. Since many galectin-like sequences (abou 10) have been found in the genome of C.elegans as a result of the genome project, these sequences were cloned from a cDNA library into an expression vector in order to confirm whether they are functional genes or not. Some sequences were shown to actually encode proteins having sugar-binding ability. It is interesting that C.elegans has as many galectins as mammals do. Glycoproteins as possible candidates for endogenous ligands for galectins have been isolated, and their structural features are now being investigated. Localization of galectins investigated by immunohistochemical technique also indicated the existence of common biological functions between C.elegans and mammals. An efficient analytical procedure based on capillary electrophoresis for comparison of functions of galectins of different origins was also developed in this research project.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Akimoto,Y.,: "″Expression of the endogenous 14-kDa beta-galactoside-binding lectin galectin in normal human skin″" Cell Tissue Res.280. 1-10 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimura,K.,: "″Determination of the affinity constants of pea lectin for neutral sugars by capillary affinophoresis with aminoligand affinophore″" J.Biochemy. 120. 1146-1152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirabayashi,J.,: "″Purification and molecular characterization of a novel 16-kDa galectin from the Nematode Caenorhabditis elegans″" J.Biol.Chem.271. 2497-2505 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arata,Y.,: "″An immunohistochemical study of the 32-kDa galectin in the nematode Caenorhabditis elegans″" Histochem.J.28. 201-207 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kasai,K.,: "″Galectins:A family of animal lectins that decipher glycocodes″" J.Biochem.119. 1-8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Walzel,H.,: "″Cell calcium signalling induced by endogenous lectin carbohydrate interaction in the Jurkat T cell line″" Glycoeonjugate J.13. 99-106 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimura, K., Kasai, K: ""Determination of the affinity constants of concanavalin A for monosaccharides by fluorescence affinity probe capillary electrophoresis"" Anal.Biochem. 227. 186-194 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akimoto, Y., Hirabayashi, J., Kasai, K., Hirano, H: ""Expression of the endogenous 14-kDa bata-galactoside-binding lectin galectin in normal human skin"" Cell Tissue Res. 280. 1-10 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akimoto, Y., Obinata, A., Hirabayashi, J., Sakakura, Y., Endo, H., Kasai, K., Hirano, H: ""Changes in expression of two endogenous bata-galactoside-binding isolectins in the dermis of chick embryonic skin during development in ova and in vitro"" Cell Tissue Res. 279. 3-12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirabayashi, J., Ubukata, T., Kasai, K: ""Purification and molecular characterization of a novel 16-kDa galectin from the Nematode Caenorhabditis elegans"" J.Biol.Chem. 271. 2497-2505 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shimura, K., Kasai, K: ""Determination of the affinity constants of pea lectin fro neutral sugars by capillary affinophoresis with aminoligand affinophore"" J.Biochem. 120. 1146-1152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arata, Y., Akimoto, Y., Hirabayashi, J., Kasai, K., Hirano, H: ""An immunohistochemical study of the 32-kDa galectin in the nematode Caenorhabditis elegans"" Histochem. 28. 201-207 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kasai, K., Hirabayashi, J: ""Glectins : A family of animal lectins that decipher glycocodes"" J.Biochem. 119. 1-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Walzel, H., Hirabayashi, J., Kasai, K., Brock, J., Neels, P: ""Cell calcium signalling induced by endogenous lectin carbohydrate interaction in the Jurkat T cell line"" Clycoconjugate J. 13. 99-106 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirabayashi,J.,: ""Purification and molecular characterization of a novel 16-kDa galectin from the Nematode Caenorhabditis elegans"" J.Biol.Chem.,. 271. 2497-2505 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Walzel,H.,: ""Cell calcium signalling induced by endogenous lectin carbohydrate interaction in the Jurkat T cell line"" Glycoconjugate J.,. 13. 99-106 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shimura,K.,: ""Determination of the affinity constants of pea lectin for neutral sugars by capillary affinophoresis with aminoligand affinophore"" J.Biochem.,. 120. 1146-1152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arata,Y.,: ""An immunohistochemical study of the 32-kDa galectin in the nematode Caenorhabditis elegans"" Histochem.J.,. 28. 201-207 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kasai,K.,: ""Galectins : A family of animal lectins that decipher glycocodes"" J.Biochem.,. 119. 1-8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshio Akimoto: "Changes in expression of two endogeneous β-galactoside-binding issleclins in the donmis of chick embryonic skin" Cell Tissue Res.279. 3-12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshio Akimoto: "Expression of the endogenous 14-kDa β-galactoside-binding lccted galectch in normal human skin." Cell Tissue Res.280. 1-10 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 平林 淳: "ガレクチン:糖鎖情報の解読者" 生化学. 67. 1366-1387 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ken-ichi Kasai: "Galectins : A family of animal lectins that decipher glycocodss" J. Biochem.119. 1-8 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jun Hirabayashi: "Durification and molealar characterization of a monel 16-kDa galection from the nematods Caenovhabditis elegans." J. Biol. Chem.271. 2497-2505 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi