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Regulatory mechanism of Ras in intracellular Signal Transduction

Research Project

Project/Area Number 07680677
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionTOKYO INSTITUTE OF TECHNOLOGY

Principal Investigator

SATO Takaya  TOKYO INSTITUTE OF TECHNOLOGY,Biosci.& Biotech., Assistant, 生命理工学部, 寄附講座教員 (20251655)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsRas / GTP-binding protein / signal transduction / IL-3 / ファルネシル化阻害剤
Research Abstract

Regulatory mechanisms and the physiological role of the Ras-mediated signaling pathway in hematopoietic BaF3 cells were analyzed. Involvement of the Ras-guanine nucleotide exchange factor mSos in IL-3-induced Ras activation was examined by the use of a stable transfectant that conditionally expresses dominant-negative mSos. We found that the dominant-negative mSos mutant inhibited Ras-regulated kinases such as ERK2, suggesting an important role of mSos in IL-3 signal transduction. Furthermore, Ras-dependent activation of JNK1 in response to IL-3 stimulation was observed. We also analyzed the roles of small GTP-binding proteins, Cdc42, Rac, and Rho in signaltransduction leading to JNK1 activation in a transient expression system. In regard with the tyrosine kinase ACK,a target of Cdc42, enhanced tyrosine phosphorylation of this protein was detected following temperature shift-down, hyperosmotic shock, and EGF treatment.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Tomokazu Ohtsuka: "Analysis of the T-cell activation signaling pathway mediated by tyrosine kinases,protein kinase C,and Ras protein,which is modulated by intracellular cyclic AMP" Biochim.Biophys.Acta. 1310. 223-232 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takaya Satoh: "Tyrosine phosphorylation of ACK in response to temperature shift-down,hyperosmotic shock,and epidermal growth factor stimulation" FEBS Lett.386. 230-234 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji Terada: "Ras-dependent activation of c-Jun N-terminal kinase/stress-activated protein kinase in response to interleukin-3 stimulation in hematopoietic BaF3 cells" J.Biol.Chem.272. 4544-4548 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomokazu Ohtsuka et al.: "Analysis of the T-cell activation signaling pathway mediated by tyrosine kinases, protein kinase C,and Ras protein, which is modulated by intracellular cyclic AMP" Biochim.Biophys.Acta. 1310. 223-232 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takaya Satoh et al.: "Tyrosine phosphorylation of ACK in response to temperature shift-down, hyperosmotic shock, and epidermal growth factor stimulation" FEBSL ett.386. 230-234 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koji Terada et al.: "Ras-dependent activation of c-Jun N-terminal kinase/stress-activated protein kinase in response to interleukin-3 stimulation in hematopoietic BaF3 cells" J.Biol.Chem.272. 4544-4548 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomokazu Ohtsuka etal.: "Analysin of the T-cell activation signaling pathway mediated by Tyrrine kinve,protein kinare c, and Riaprotein mknch is modnlated by intracellntar cycl AMP." Biochim,Biophya,Acta. 1310. 223-232 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takaya Satoh et al.: "Tyrnine phorgharylation of ACK in rexpometo Tem peratme shift-drwn,hyperomotic shock,and epidermak gnowth faiten stimulation" FEBS Lett.386. 230-234 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koji Terada et al.: "Ras-deoendent activation of c-Jun N-temonal kijara/strese-acivated protein kinare in rerpunse to intalenkis -3 at imulat in heimasto poietic Bai3 cells." J.Bial.Chem.272. 4544-4548 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiko Ito et al.: "G protein β γ subunit a ctivutesRas.Rat,and MAP kinare in HEK 293 cells" FEBS Lett.368. 183-187 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Koji Terada et al.: "Ras in not reguired for the interleukin3-induced prohteratrin of a moure pro-B cell line,BaF3" J.Biol.Chem. 270. 27880-27886 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sadao Man et al.: "Inhibition of Ras.Rat interaction by anti-one ogenic must ants of nernafibuomin,the nemafibromatos type I(NF1)gene poduct,in a celt free syoeleme" J.Biol.Chem.270. 28834-28838 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sadao Man et al.: "Inhibition of Ras.Rat interaction by anti-one ogenic must ants of nunafibiomin,the nemafibromatos type I(NF1)gene poduct" J.Biol.Chem. 270. 28834-28838 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tomokazu Ohtsuka et al: "Analysin of the T-cell caetivctrim signaluepathucy mechiated by tyramiknin protein kynine C,and Ras protein mhich in modulcted by introcelluln cyclic Amp." Biochein,Bioplys,Acta.(印刷中). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takuya Satoh et al.: "Meucmement of Ras-bound guanine nucleotide in stimulated hematopoietic cells." Methods Engymal.255. 149-155 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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