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RNA binding protein regulated differentiation- dependent alternative splicing

Research Project

Project/Area Number 07680685
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

NAKAMURA Masahiko  Institute for Protin Research, Osaka University, Instructor, たんぱく質研究所, 助手 (20172439)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordssplicing / C2C12 / myoblast / RNA binding protein / SR protein / myotube / intron / PCR / SR-pretein
Research Abstract

Alternative splicing is a comon mechanism for the generation of multiple mRNAs with different stabilities, translation efficiencies, or protein coding sequences from a single gene. The selection of an alternative exon is often under the control of cell type-specific or differentiation state-dependent splicing machinery. The efficiency of exon selection is determined by a balance of several determinants, such as splice donor and acceptor site sequences, exon size, enhancer and repressor resided in exons and intron, and relative potentials of competing splice site sequences. Most of the cis-acting regulatory elements characterized up to date involved in a constitutive splicing pathway. In mammalian genes, only a limited number of cis-elements that participate in the alternative splicing has been reported. We found for an intronic cis-acting element located downstrem exon MSDb (muscle-specific domain b) in the NCAM (neural cell adhesion molecule) gene. The cis-element acted as a negative regulator for the selection of exon MSDb in nonmuscle fibroblast but not in myoblasts, that are already destined to differentiate into muscle cells. The suppressive effect of this cis-element on the selection of exon MSDb was released in the process of myogenesis. We searched for trans-acting factor (s) that bind to the cisacting elements, such as splicing factors and regulators.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] N.Hamai, M.Nakamura & A.Asano: "Inhibition of mitochondrial protein synthesis impaired C2C12 myoblast differentiation" Cell Structure and Function. 22. 421-431 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Kawahigashi, Y.Harada, M.Nakamura & A.Asano: "A cis-acting regulatory elemment that affects the alternative splicing of a muscle-specific exon in the mouse NCAM gene" Biochimica Biophysica Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Hamai.M.Nakamura & A.Asano: "Inhibition of mitochondrial protein synthesis impaired C2C12 myoblast differentiation" Cell Structure and Function. 22. 421-431 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Kawahigashi, Y.Harada, M.Nakamura & A.Asano: "A cis-acting regulatory elemment that affects the alternative splicing of a muscle -specific exon in the mouse NCAM gene" Biochimica Biophysica Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Hamai, M.Nakamura & A.Asano: "Inhibition of mitochondrial protein synthesis impaired C2C12 myoblast differentiation" Cell Structure and Function. 22. 421-431 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Kawahigashi, Y.Harada, M.Nakamura & A.Asano: "A cisacting regulatory elemment that affects the alternative splicing of a musclespecific exon in the mouse NCAM gene" Biochimica Biophysica Acta. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Y.Harada,M.Nakamura & A Asano: "Temporally distinctive changes of altemative splicing patterns during myogenic differentiation of C2C12 cells" J.Biochem.118. 780-790 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yoshimi,M.Nakamura & A,Asano: "Studies on differentiation mechanisms from pineal body cells to muscle cells(II)" Cell Structure and Function. 20. 588- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yoshimi,M.Nakamura & A,Asano: "Expression of seveal muscle-specific genes during differntiation of cultured pineal body cells under artificial (high NaCl)conditions" Differentiation. 59. 307-319 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Harada,M.Nakamura & A.Asano: "Temporally distinctive changes of alternative splicing pattems dunng myogenic differentiation of C2C12 cells" J.Biochem.118. 780-790 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Yoshimi,M.Nakamura & A.Asano: "Studies on differentiation mechanisms from pineal body cells to muscle cells (II)" Cell Structure and Function. 20. 588 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Yoshimi,M.Nakamura & A.Asano: "Expression of several muscle-specific genes duning differentiation of cultured pineal body cells under artificial (high NaCl) conditions" Differentiation. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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