Compilation of the vibosome-related protein seguences and its application to the analysis on eukavyotic cellular evalution
| Project/Area Number |
07680742
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | THE INSTITUTE OF STATISTICAL MATHEMATICS |
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Principal Investigator |
HASHIMOTO Tetsuo The Institute of Statistical Mathematics, Statistical Education and Information Center, Associate Professor, 統計教育・情報センター, 助教授 (50208451)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUTA Keiko Research Institute for Radiation Biology and Medicine, Hiroshima University, Dev, 原爆放射能医学研究所, 助手 (40166012)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | ribosomal protein / molecular Evolution / alignment / molecular phylogenetic tree / eukaryotic cell / 下等真核生物 |
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| Research Abstract |
Large number of ribosomal protein (r-protein) sequences form various organisms have been accumulated up to now in the sequence databases. Although these data might have many informations on the evolution of the ribosomes, these are not well arranged in order because nomenclatures are various among different organisms, In this work we have systematically compiled the r-protein species, evolutionarily analyzing sequence similarities.
On the basis of all the alignments constructed and phylogenetic analyzes carried out up to now, the evolutionary profile of r-proteins is emerging : (1) in no small number there are protein species that exist throughout the three primary kingdoms, (2) almost all the alignments show 'one to one' correlation to align regularly, that is irregular correlation such as internal transposition appears to be rarely found, (3) wide insertion/deletions and elongation in length are usually found in the comparisons among sequences from different primary kingdoms, while within a kingdom most of the evolutionary changes are amino acid replacements and minor insertion/deletions, (4) gene duplication events have occurred on several r-protein species in the various stages of eukaryotic evolution, (5) archaebactria do not show a monophyletic clade in no small number of r-protein species.
On the other hand, we began to analyze the genes of ribosome-related molecules from amitochondriate protists, Glugea plecoglossi, Giardia lamblia and Trichomonas vaginalis to elucidate early evolution of eukaryotic ribosomes.
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Report
(3 results)
Research Products
(10 results)
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[Publications] Kamaishi,T.,Hashimoto,T.,Nakamura,Y.,Masuda,Y.,Nakamura,F.,Okamoto,K.,Shimizu,M.and Hasegawa,M.: "Complete nucleotide sequences of the genes encoding translation elongation factors 1α and 2 from a microsporidian parasite,Glugea plecoglossi: implications for the deepest branching of eukaryotes." Journal of Biochemistry. 120. 1095-1103 (1996)
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[Publications] Yamamoto,A.,Hashimoto,T.,Asaga,E.,Hasegawa,M.and Goto,N.: "Phylogenetic position of mitochondrion-lacking protozoan,Trichomonas tenax,based on amino acidsequences of elongation factors 1α and 2." Journal of Molecular Evolution. 44. 98-105 (1997)
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[Publications] Mizuta,K.,Park,J.S.,Sugiyama,M.,Nishiyama,M.and Warner,J.R.: "RIC1,a novel gene required for ribosome synthesis in Saccharomyces cererisiae." Gene, inpress.
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