• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

IDENTIFICATION AND ROLE OF CYTOSOLIC FACTORS THAT PARTICIPATE IN TRANSLOCATION OF LYSOSOMAL MEMBRANE PROTEINS

Research Project

Project/Area Number 07680770
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TANAKA Yoshitaka  KYUSHU UNIVERSITY,FACULTY OF PHARMACEUTICAL SCIENCES,INSTRUCTOR, 薬学部, 助手 (20217095)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsLYSOSOME / ENDOSOME / SORTING SIGNAL / ソーティング・シグナル
Research Abstract

The aim of this study is to identify cytosolic factors that recognize the cytoplasmic domain of lysosomal membrane proteins and mediate transport to the lysosomes, and further to elucidate molecular mechanisms of cytosolic factors in sorting pathway of lysosomal membrane proteins.
When the cytosol fraction prepared from rat livers was applied to the column conjugated fusion proteins of GST and cytoplasmic domain of lysosomal membrane protein, LGP85, cytosolic factors were eluted with 0.5M NaCl. From analysis by SDS-PAGE,proteins having molcularweight of 90,000 and 53,000 were identified. In this purification step, the presence of cytosolic factors that bind to cytoplasmic domain of LGP85 was assayd by dot blotting using horseradish peroxidase-conjugated synthetic peptides against cytoplasmic domain of LGP85 (HRP-LGP85C-tail). It was found that binding of cytosolic factors and HRP-LGP85C-tail is specific, because this binding was inhibited in the presence of excess amounts of synthetic peptides.
When analyzed N-terminal sequences of both CF90 and CF53, CF90 and CF53 were determined N-terminal sequences of 16 amino acid residues and 11 amino acid residues, respectively. Furthermore, it was found that N-terminal sequences of CF90 were identical with those of heat shock protein 90 (HSP90), and N-terminal sequences of CF53 represented highly homology to those of beta-tubulin. This was further confirmed by immunoblotting analysis using specific antibodies against HSP90 or beta-tubulin.
Mechanisms by which HSP90 and beta-tubulin participate in lysosomal targeting and function in lysosomes of LGP85 are examining at present.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 田中嘉孝: "ゴルジ体以降の選別" 日経サイエンス. 25・3. 41-50 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 田中嘉孝: "ライソゾーム蛋白質の選別・輸送" 生体の化学. 46・12. 204-209 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 姫野勝: "リソソーム酵素の構造とその受容体" 日本臨床. 53・12. 2892-2897 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 姫野勝: "蛋白質生合成過程、翻訳後プロセシングとリソソームへの輸送機構" 日本臨床. 53・12. 2898-2903 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YOSHITAKA,TANAKA: "SORTING FROM GOLGI APPARATUS" NIKKEI SCIENCE. VOL.25, NO.3. 41-50 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YOSHITAKA,TANAKA: "SORTING AND TRANSPORT OF LYSOSOMAL PROTEINS" SEITAINOKAGAKU. VOL.46, NO.3. 204-209 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MASARU,HIMENO: "LYSOSOMAL HYDROLASES HAVE SPECIFIC CONFORMATIONAL DOMAINS FOR ACQUISITION OF MANNOSE-6-PHOSPHATE" NIHONRINSHO. VOL.53, NO.12. 2892-2897 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MASARU,HIMENO: "BIOSYNTHESIS PROCESSING,AND LYSOSOME TARGETING OF ACID PHOSPHATASE" NIHONRINSHO. VOL.53, NO.12. 2898-2903 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 田中嘉孝: "ゴルジ体以降の選別" 日経サイエンス. 25. 41-50 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中嘉孝: "ライソゾーム蛋白質の選別・輸送" 生体の化学. 46. 204-209 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 姫野勝: "リソソーム酵素の構造とその受容体" 日本臨床. 53. 2892-2897 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 姫野勝: "蛋白質生合成過程、翻訳後プロセシングとリソソームへの輸送機構" 日本臨床. 53. 2898-2903 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中嘉孝: "ゴルジ体以降の選別" 日経サイエンス. 25. 41-50 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田中嘉孝: "ライソゾーム蛋白質の選別・輸送" 生体の化学. 46. 204-209 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi