| Project/Area Number |
07680778
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
KIHARA Hiroshi Kansai Medical University, Medicien, Professor, 医学部, 教授 (20049076)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOYO Akitsugu Kansai Medical University, Medicien, Assistant Professor, 医学部, 講師 (30174775)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | x-ray microscopy / zone plate / synchrotron radiation / intracellular structure / endocytosis / immunogold / 結像型軟X線顕微鏡 / シンクロトロン放射光 / ナノイメージング / UVSOR / in vivo観察 / ラベリング / 冷却CCDカメラ / in vivo 細胞観察 / ホヤの血液細胞 |
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| Research Abstract |
The purpose of the present research is to observe intracellular structure of the cells and to search specific labeling techniques of the specific site for monitoring by x-ray microscopy.
The x-ray microscope used in the present research is firstly set up at UVSOR,and then moved to RITS SR center. A part of the present works were done at BESSY (Berlin).
1) Construction of the x-ray station. We have been improving the x-ray station by ; (1) To observe the same specimens by the light microscope as well as by x-ray microscope. (2) To set specimens in the air. The detector was changed to cooled CCD to improve its quantum efficiecy. With this syustem ; we have observed many biospecimens such as diatoms, blood cells of ascidian, clamydomonas, muscle fibers, tumor cells, protoplasts and chromosomes both in wet and dried states.
2) Analysis on the intracellular structure and intracellular transport by specific lableing techniques. On this subject, we have done 3 studies. (1) By staining microtubles with Au-labeled antiubodies, we observed cytoskeltons. (2) COS cells were stained by potassium permanganate and observed in wet state. (3) Analysis of endocytosis by x-ray microscopy. All of the three researches demonstrate that specific labeling is very useful in elucidating intracellular structure and its related functions in wet state.
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