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Identification of genes expressed in Drosophila germline

Research Project

Project/Area Number 07680793
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KOBAYASHI Satoru  University of Tsukuba, Inst.Biological Sciences, Assistant Professor, 生物科学系, 講師 (90225508)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsDrosophila / Pole Cell / Germline / Enhancer-trap / Differential Display / Polar granules / 生殖系列 / nanos遺伝子
Research Abstract

In many animal groups, factors required for germline formation are localized in a histologically remarkable region in egg cytoplasm, called germ plasm. In Drosophila embryos, germ plasm is localized in the posterior pole region and is inherited in pole cells, the germ-line progenitors in this animal. Transplantation experiments have demonstrated that germ plasm contains factors with sufficient ability to form germ line. Germ plasm also contains a second localized activity that directs the formation of abdomen. Genetic analysis has revealed that a common mechanism directs the localization of the abdomen and germ-line forming factors to the posterior pole. The critical factor for abdomen formation is the product of the nanos (nos) gene.
In this study, we show that nos is also esential for germ-line formation in Drosophila. Pole cells lacking nos activity are incapable of becoming functional germ cells because they fail to migrate into the gonads. In such pole cells, enhancer activation which normally initiates within the gonad begins prematurely during pole cell migration. Premature activation of genes in germline precursors may cause these cells to be incapable of following the normal germline developmental program.
Furthermore, we have identified an untranslatable RNA,called Pgc, which is localized in germ plasm. Most pole cells in embryos produced by females expressing anti-sense Pgc RNA fail to complete migration and to populate the embryonic gonads, and females which develop from these embryos often have agametic ovaries. Our results support an essential role for Pgc RNA in germ line development.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] S.Kobayashi: "Essential role of the posterior morpnogen nanos for germline development in Drosophila" Nature. 380. 708-711 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Nakamura: "Requirement for anoncoding RNA in Drosophila polargranulos for germ cell establishment." Science. 274. 2075-2079 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kobayashi, M.Yamada, M.Asaoka and T.Kitamura: "Essential role of the posterior morphogen nanos for germline development in drosophila" Nature. 380. 708-711 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Nakamura, R.Amikura, M.Mukai, S.Kobayashi and P.F.Lasko: "Requirement for a noncoding RNA in Drosophila polar granules for germ all establishment" Science. 274. 2075-2079 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kobayashi: "Essential role of the posterior morphogen nanos for germline development in drosophila" Nature. 380. 708-711 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Nakamura: "Requirement for a noncoding RNA in Drosophila polar gramules for germ cell establishment" Science. 274. 2075-2079 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Kobayashi: "Mislocalization of oskar product in the anterior pole results in ectopic localization of mitochondrial large rRNA in Drosophila embryos." Developmental Biology. 169. 384-386 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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