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Study of the receptor of exogastrula-inducing peptides

Research Project

Project/Area Number 07680794
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionSaitama University

Principal Investigator

SUYEMITSU Takashi  Saitama Univ, Fac.Sci.Dpt.Reg.Biology, Professor, 理学部, 教授 (40092019)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsExogastrula / gastrulation / Sea urchin / Exogastrula inducing peptides / Gastrulation / Exogastrula-indacing peprides / ウニ / 外腸胚 / 外腸胚形成ペプチド / 原腸形成 / 表皮成長因子
Research Abstract

In order to elucidate the function of exogastrula-inducing peptides (EGIPs), EGIP-binding protein (EBP) was examined in this study. At first, EBP was purified and its N-terminal amino acid sequences were determined. The cDNA fragments of EBP were amplified by RT-PCR method using degenerate primers. Two cDNA clones, that is, EBP-alpha and EBP-beta were obtained from a cDNA library of unfertilized eggs of the sea urchin Anthocidaris crassispina using, as probe, the cDNA fragments obtained by the RT-PCR method. The base sequences of the two cDNA clones were very similar to each other (96% identity). Moreover, the two clones showed significant similarity to the extracellular matrixes of the other sea urchin embryos, that is, HLC-32 and bep4.
I examined the level of EBP-alpha mRNA during the development of sea urchin embryos. It became clear that EBP-alpha mRNA was present in unfertilized eggs. The level of the mRNA decreased gradually as development progressed to the pluteus stage, although there was a transient increase of the mRNA level at the prism stage. The recombinat EBP-alpha expressed in E.colibound to EGIP and inhibited the exogastrula-iunducing activity of EGIP.These results suggest that EBP-alpha is the binding protein of EGIP.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Y.Fujita: "Purificatin of EGIP-D-binding protein from embryos of the sea urchin Anthocidarrs crassispina." Zoological Science. 14. 931-934 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sakuma: "The protein tyoosine kinases of the sea urchin Anthocidares crassisping" Zoological Science. 14. 941-946 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yamasu: "Moleular cloning of a cDNA that encodes the precursor to several exogastrula-inducing peptides,EGF-related poly peptides of the seur clin AC" Eur.J.Biochem.228. 515-523 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Ymasu, H.Watanabe, C.Kohchi, G.Soma, D.Mizuno, K.Akasaka, H.Shimada, T.Suyemitsu and K.Ishihara: "Molecular cloning of a cDNA that encodes the precursor to several exogastrula-inducing peptides, epidermal-growth-factor-related polypeptides of the sea urchin Anthocidaris crassispina." Eur.J.Biochem.228. 515-523 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Fujita, Y.Hirate, T.Hiyama, K.Yamasu, T.Suyemitsu and K.Ishihara: "Purification of EGIP-D-binding protein from the embryos of the sea urchin Anthocidaris crassispina." Zool.Sci.14. 931-934 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Sakuma, H.Onodera, T.Suyemitsu and K.Yamasu: "The protein tyrosine kinases of the sea urchin Anthocidaris crassispina." Zool.Sci.14. 941-946 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Fujita: "Purification of EGIP-D-binding protein from embryos of the sea urchin Antheocidaris crassispina." Zoological Science. 14. 931-934 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Sakuma: "The protein tyrosine kinases of me The sea urchin Antheocidaris crassispina." Zoological Science. 14. 941-946 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Sakuma: "Molecalar cloning of a DNA rnat encadees the precursor to sevenol Rtogastrula-inducing pertides EGF-relaed paly peptides of the sea cechin,A.C." Ecer,J,Biochem.228. 515-523 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.P.Binette: "The content and distribution of glycosaminoglycans in the ejaculates of normal and vasectomized men" Andrologia. 28. 145-149 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Yamasu: "Molecular cloning of a cDNA that encodes the precursor to several EGIPS、EGF-related polypeptides of the sea urchin Anthocidaris crassispina." Eur. J. Biochem.228. 515-523 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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