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Mechanisms of regeneration of insect imaginal discs.

Research Project

Project/Area Number 07680795
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionThe University of Tokyo

Principal Investigator

KUBO Takeo  The University of Tokyo, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10201469)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Ayako  The University of Tokyo, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助手 (90272484)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsSarcophaga peregrina / Imaginal discs / Regeneration / Ecdyson / Periplaneta americana / Lectin / Regenectin / 26-kDa lectin / 昆虫 / 遺伝子発現 / cDNAクローニング / 分子進化 / in vitro / エクダイソンレセプター / wingless
Research Abstract

1. Restoration of positional values of Sarcophaga imaginal discs on regeneration in vitro.
When the 1/4 sectors of imaginal discs of Sarcophaga expressing wingles (wg) gene are removed and the remeining parts are cultured in the presence of 2.5 x 10^<-8>M 20-hydroxyecdyson, restoration of their morphology occurrs. Here, we isolated a cDNA for the Sarcophaga wg gene, and analyzed whether the lost positional values revealed by wg expression were restored when the remaining parts of imaginal discs were cultured in the presence of 20-hydroxyecdyson. As results, expression of the wg gene was detected in the regenrated areas, indicating that regeneration of imaginal discs in vitro minics in vivo regeneration in that it involves restoration of positional values.
2.cDNA cloning and analysis of expression of the gene for lectins that localize in the regenerating legs of American cockroach.
Two humoral lectins, termed 26-kDa lectin and regenectin, appear transiently in the regenerating legs of Amer … More ican cockroach. Among them, 26-kDa lectin localizes around epidermis of the regenerating legs in the middle stage of regeneratin, whereas regenectin localizes around regenetating muscle cells in the late stage. In this study, we isoalted cDNAs for these lectins and determined their primary structures. As results, these lectins were found to be C-type (Ca^<2+>-dependent) lectins and shares about 50% sequence identities with eact other. these results suggest that these lectins belong to the same protein family and play different roles in leg regeneration. The gene for regenectin was found to express transiently in the regenerating epidermis, suggesting that it functions in the formation of muscle cells in a paracrine manner by synthesized and secreted by the epidermis.
This is the first instance that identified molecules (20-hydroxyecdyson and humoral lectins) that is involved in the regeneration in insects and woud be an important clue to better undrstand the molecular mechanisms of organ regeneration. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Sung Ook Chung: "Molecular cloning and sequencing of arylphorin-binding protein in protein granules of the Sarcophaga fat body." J.Biol.Chem.270. 4624-4631 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toshiyuki Nakanishi: "Structure-function relationship of yeast S-II in terms of stimulation of RNA polymerase II,arrest relief,and supression of 6-azauracil sensitivity" J.Biol.Chem.270. 8991-8995 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mayumi N.-Matsui: "Molecular cloning and nuclear localization of ATBP,a novel (A+T) -stretch-binding protein of Sarcophaga peregrina (flesh fly)." Eur.J.Biochem.230. 396-400 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeo Kubo: "Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role." J.Biochem.119. 291-295 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuaki Ohashi: "Molecular cloning of cDNA and analysis of expression of the gene for α-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L." Biochem.Biophys.Res.Commun.221. 380-385 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahiro Ito: "Spermatocyte-specific expression of the gene for mouse testis-specific transcription factor S-II." FEBS Lett.385. 21-24 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kiyoshi Kawasaki: "Presence of the Periplanata lectin-related protein family in the American cockroach Periplanata amaricana." Insect Biochem.Mol.Biol.26. 355-364 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomohiro Horio: "Purification and cDNA cloning of the alcohol dehydrogenase of the flesh fly Sarcophaga peregrina." Eur.J.Biochem.237. 698-703 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Safiqul Haq: "Purification,characterization,and cDNA cloning of a galactose-specific C-type lectin from Drosophila melanogaster." J.Biol.Chem.271. 20213-20218 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nobuaki Adachi: "Purification,characterization and cDNA cloning of ABP-2 (Arylphorin gene-specific binding protein-2) that specifically binds to the ABP-1-binding sequence in the arylphorin gene of Sarcophga peregrina." J.Biochem.120. 1239-1246 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeo Kubo: "Insect lectins and epimorphosis" Trends in Glycosci.Glycotech.8. 357-364 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jae Yoon Leen: "Purification and characterization of N-β-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanin,a novel antibacterial substance of Sarcophaga peregrina (flesh fly)." J.Biol.Chem.271. 13573-13577 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamaki Yano: "Purification and characterization of cathepsin B mRNA 3′-untranslated-region-binding protein (CBBP),a protein that supresses cathepsin B mRNA translation." Eur.J.Biochem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takekazu Kunieda: "Regeneration of Sarcophaga imaginal discs in vitro : implication of 20-hydroxyecdyson." Devel.Biol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunji Natori: "Role of lectins in development and merphogenesis in insects. In New Directions in Invertabrate Immunology (K.Soderhall et al eds.)" SOS Publications, 12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sung Ook Chung, Takeo Kubo, and Shunji Natori: "Molecular cloning and sequencing of arylphorin-binding protein in protein granules of the Sarcophaga fat body." J.Biol. Chem.270. 4624-4631 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toshiyuki Nakanishi, Makoto Shimoaraiso, and Shunji Natori: "Structure-function relationship of yeast S-II in terms of stimulation of RNA polymerase II,arrest relief, and supression of 6-azauracil sensitivity." J.Biol. Chem.270. 8991-8995 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mayumi Nakanishi-Matsui, Takeo Kubo, and Shunji Natori: "Molecular cloning and nuclear localization of ATBP,a novel (A+T)-stretch-binding protein of Sarcophaga peregrina (flesh fly)." Eur. J.Biochem.230. 396-400 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeo Kubo, Masami Sasaki, Jun Nakamura, Hiromi Sasagawa, Kazuaki Ohashi, Hideaki Takeuchi, and Shunji Natori: "Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role." J.Biochem.119. 291-295 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuaki Ohashi, Miyuki Sawata, Hideaki Takeuchi, Shunji Natori, and Takeo Kubo: "Molecular cloning of cDNA and analysis of expression of the gene for a-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L." Biochem. Biophys. Res. Commun.221. 380-385 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahiro Ito, Qihong Xu, Hideaki Takeuchi, Takeo Kubo, and Shunji Natori: "Spermatocyte-specific expression of the gene for mouse testis-specific transcription factor S-II." FEBS Lett.385. 21-24 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kiyoshi Kawasaki, Takeo Kubo, and Shunji Natori: "Presence of the Periplanata lectin-related protein family in the American cockroach Periplanata amaricana." Insect Biochem. Mol. Biol.26. 355-364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomohiro Horio, Takeo Kubo, and Shunji Natori: "Purification and cDNA cloning of the alcohol dehydrogenase of the fkesh fly Sarcophaga peregrina." Eur. J.Biochem.237. 698-703 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Safiqul Haq, Takeo Kubo, Shoichiro Kurata, Ayako Kobayashi, and Shunji Natori: "Purification, characterization and cDNA cloning of a galactose-specific C-type lectin from Drosophila melanogaster." J.Biol. Chem.271. 20213-20218 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nobuaki Adachi, Takeo Kubo, and Shunji Natori: "Purification, characterization, and cDNA cloning of ABP-2 (Arylphorin gene-specific binding protein-2) that specifically binds to the ABP-1-binding sequence in the arylphorin gene of Sarcophga peregrina." J.Biochem.120. 1239-1246 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeo Kubo, Kiyoshi Kawasaki, and Shunji Natori: "Insect lectins and epimorphosis" Trends in Glycosci. Glycotech.8. 357-364 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jae Yoon Leem, Chiaki Nishimura, Shoichiro Kurata, Ichiro Shimada, Ayako Kobayashi, and Shunji Natoril: "Purification and characterization of N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxy-phenylalanin, a novel antibacterial substance of Sarcophaga peregrina (flesh fly)." J.Biol Chem. 271. 3573-13577 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamaki Yano, Ayako Kobayashi, Shoichiro Kurata and Shunji Natori: "Purification and characterization of cathepsin B mRNA 3'-untranslated-region-binding protein (CBBP), a protein that supresses cathepsin B mRNA translation." Eur. J.Biochem. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takekazu Kunieda, Shoichiro Kurata and Shunji Natori: "Regeneration of Sarcophaga imaginal discs in vitro : implication of 20-hydroxyecdyson." Devel. Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunji Natori and Takeo Kubo: Role of lectins in development and merphogenesis in insects.In New Directions in Invertabrate Immunology (K.Soderhall et al eds.) SOS Publications, 1996

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kawasaki,K.: "Presence of the Periplanata lectin-related protein family in the American cockroach Periplanata americana." Insect Biochem.Molec.Biol.26. 355-364 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kubo,T.: "Insect lectins and epimorphosis" Trends in Glycosci.Glycotech.8. 357-364 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kubo,T.: "Change in the expression of the hypopharyngeal gland proteins of the worker honeybee(Apis mellifera L.)with age and/or role." J.Biochem.119. 291-295 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohashi,K.: "Molecular cloning of cDNA and analysis of expression of the gene for α-glucosidase from the hypopharyngeal gland proteins of the worker honeybees Apis mellifera L." Biochem.Biophys.Res.Commun.221. 380-385 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Horio,T.: "Purification and cDNA cloning of the alchol dehydrogenase of the flesh fly Sarcophaga peregrina" Eur.J.Biochem.237. 698-703 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Haq,S.: "Purification,characterizafion,and cDNA cloning of a galactose-specitic C-type lectin from Drosophila melanogaster." J.Biol.Chem.271. 20213-20218 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Natori,S.: "New directions in insect immunity" Role of lectins in development and morphogenesis in insects., 13 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Chung,S.-O.et al.: "Molecular cloning and sequencing of arylphorin-binding protein in protein granules of the Sarcophaga peregrina." J.Biol.Chem.270. 4623-4631 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakanishi,T.et al.: "Structure-function relationship of yeast S-II,in terms of stimulation of RNA polymerase II,arrest relief,and suppression of 6-azauracil sensitivity." J.Biol.Chem.270. 8991-8995 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsui,N.M.et al.: "Molecular cloning and nuclear localization of ATBP,a novel (A+T)-stretch-binding protein of Sarcophaga peregrina (flesh fly)." Eur.J.Biochem.230. 396-400 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kubo,T.et al.: "Change in the expression of hypopharyngeal-gland proteins of the worker honeybee (Apis mellifera L.) with age and/or role." J.Biochem.119(in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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