MOLECULAR ANALYSIS OF ABDOMINAL LEG DEVELOPMENT IN MUTANTS IN THE E COMPLEX OF BOMBYX MORI
| Project/Area Number |
07680809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | OKAZAKI NATIONAL RESEARCH INSTITUTES |
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Principal Investigator |
UENO Kohji NATIONAL INSTITUTE FOR BASIC BIOLOGY,ASSOCIATED PROFESSOR, 基礎生物学研究所, 助教授 (10143504)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | HOMEOTIC GENE / MORPHOGENESIS / BOMBYX MORI / 形態形成 |
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| Research Abstract |
The E complex is a homeotic gene complex that specifies abdominal segments in Bombyx mori. Analyzing the E^N and E^<Ca> chromosomes I have suggested that the E complex consists of the Bm Ubx, abd-A and Abd-B genes.
I am focusing to study the developmental mechanism of embryonic abdominal legs. Since I elucidated that the Bm abd-A region is deleted in the E^<Ca> chromosome and homozygous E^<Ca>/E^<Ca> embryos do not form abdominal legs, I speculate that the Bm abd-A gene is responsible for the development of abdominal legs.
I have isolated each cDNAs of the Bm Ubx, abd-A and Abd-B genes and prepared antibodies against the Bm Ubx, abd-A and Abd-B gene products. I have analyzed expression of Bm Ubx, abd-A and Abd-B gene products in embryo homozygous for E^<Kp> (Kp supernumerary legs), which reveals supernumerary abdominal legs on the abdminal second (A2) segment. I found that much amount of the Bm abd-A proteins were detected in A2 segments in the embryos homozygous for E^<Kp>. Therefore I speculated that the abnormal expression of the Bm abd-A proteins might cause development of extra abdminal legs on the A2 segment.
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Report
(3 results)
Research Products
(6 results)
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[Publications] Nagata, T., Suzuki, Y., Ueno, K., Kokubo, H., Xu, X., Hui, C.-c.Hara, W.and Fukuta, M.: "Developmental expression of the Bombyx Antennapedia homologue and homeotic changes in the Nc mutant" Genes to Cells. 1. 555-568 (1996)
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